Delta-G-VSV Pseudotyping System

The glycoprotein (G protein) of VSV is responsible for the attachment and entry of VSV into a susceptible host cell and is therefore essential for virus infectivity. To identify and dissect the signals required for the replication and assembly of VSV Dr. Michael Whitt has developed a "reverse genetics" system in which the G protein of VSV has been deleted (rVSV-ΔG). rVSV-ΔG has been used to produce VSV pseudotypes containing the envelope glycoproteins of heterologous viruses including viruses that require high-level containment (e.g., encephalitis viruses, SARS coronavirus, SARS-CoV-2, MERS coronavirus, etc.). Since the infectivity of rVSV-ΔG is restricted to a single round of replication, analyses of viral entry can be performed using just biosafety level 2 (BSL-2) containment.

This Delta-G-VSV Pseudotyping System has proven useful for identifying cellular receptors for viruses, screening for entry inhibitors, and evaluating neutralizing antibody responses following vaccination. Originally described in a 2010 methods article (PMID: 20709108), the system is now used as a virus model system by researchers worldwide. Most recently, the system is being applied to study COVID-19 infection.

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Cell Line 
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ΔG- DsRed 
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ΔG-GFP 
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ΔG-Luciferase 
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ΔG-N/P-MCS2-2.6 
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ΔG-P/M-MCS2-2.6 
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ΔG-PL 2.5 
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rVSV-ΔG-GFP-2.6 
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VSV large polymerase subunit (L) 
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VSV nucleocapsid (N) protein 
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VSV nucleocapsid (N) protein; VSV phosphoprotein (P); VSV large polymerase subunit (L); VSV-Indiana glycoprotein (G) 
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VSV phosphoprotein (P) 
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VSV-ΔL-GFP 
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VSV-9.1(+) 
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VSV-G (San Juan, Indiana serotype) 
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VSV-Indiana glycoprotein (G) 
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