The plasmid pBS-N-ΦT encodes the vesicular stomatitis virus (VSV) nucleocapsid (N) protein expressed from the bacteriophage T7 promoter in pBS, which has been further modified to contain a T7 terminator sequence cloned between the SacII and SacI restriction sites in pBS-SK+ [1]. The plasmid is used as part of the VSV reverse genetics system to recover recombinant VSV from plasmids [2, 3]. The recovery of rVSV involves the transfection of vaccinia-T7 infected cells with 4 (or 5) different plasmids; one that expresses the rVSV antigenome (e.g. pVSV-FL+(2) or pVSV-ΔG-GFP) and 3 (or 4) other plasmids that express the VSV N, P, and L proteins (or N, P, L and G for replication-restricted ΔG-VSV).
From the laboratory of Michael A. Whitt, Ph.D., University of Tennessee.
Part of The Investigator's Annexe program.
Product Type: | Plasmid |
Gene/insert name: | VSV nucleocapsid (N) protein |
Accession ID: | P03521 |
Antibiotic Resistance: | Ampicillin or Kanamycin (please see vial label and packing slip) |
Fusion Tag(s): | none |
Concentration: | 10uL (1ug/uL) |
Grow in E. coli at 37 C: | Yes |
Cloning Site 5': | XhoI |
Cloning Site 3': | EcoRI |
Insert Size: | 1,372 bp |
Vector Backbone and Size: | pBS-SK-ΦT, 3105 bp |
High or low copy: | High |
Comments: | For suggested protocol, see: Whitt, MA, J. Virol. Methods, 2010. 169(2): p. 365-74. |
Shipped: | Ambient temperature |
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