Set of Helper Plasmids (VSV-N, VSV-P, VSV-L, VSV-G)
The recovery of rVSV involves the transfection of vaccinia-T7 infected cells with 4 (or 5) different plasmids; one that expresses the rVSV antigenome (e.g. pVSV-FL+(2) or pVSV-ΔG-GFP) and 3 (or 4) other plasmids that express the VSV N, P, and L proteins (or N, P, L and G for replication-restricte ΔG-VSV).
From the laboratory of Michael A. Whitt, Ph.D., University of Tennessee.
Part of The Investigator's Annexe program.
The recovery of rVSV involves the transfection of vaccinia-T7 infected cells with 4 (or 5) different plasmids; one that expresses the rVSV antigenome (e.g. pVSV-FL+(2) or pVSV-ΔG-GFP) and 3 (or 4) other plasmids that express the VSV N, P, and L proteins (or N, P, L and G for replication-restricte ΔG-VSV).
From the laboratory of Michael A. Whitt, Ph.D., University of Tennessee.
Part of The Investigator's Annexe program.
Specifications
| Product Type: | Plasmid |
| Gene/insert name: | VSV nucleocapsid (N) protein; VSV phosphoprotein (P); VSV large polymerase subunit (L); VSV-Indiana glycoprotein (G) |
| Antibiotic Resistance: | Ampicillin or Kanamycin (please see vial label and packing slip) |
| Fusion Tag(s): | none |
| Concentration: | 100uL (100ng/uL) |
| Grow in E. coli at 37 C: | Yes |
| Vector Backbone and Size: | pBS-SK-ΦT, 3105 bp |
| High or low copy: | High |
| Comments: | For suggested protocol, see: Whitt, MA, J. Virol. Methods, 2010. 169(2): p. 365-74. |
| Shipped: | Ambient temperature |
Provider
From the laboratory of Michael A. Whitt, Ph.D., University of Tennessee.
References
- Stillman, E.A., J.K. Rose, and M.A. Whitt, Replication and amplification of novel vesicular stomatitis virus minigenomes encoding viral structural proteins. J. Virol., 1995. 69: p. 2946-2953.
- Lawson, N.D., et al., Recombinant vesicular stomatitis viruses from DNA. Proc.Natl.Acad.Sci.(USA), 1995. 92(10): p. 4477-4481.
- Whitt, M.A., Generation of VSV pseudotypes using recombinant DeltaG-VSV for studies on virus entry, identification of entry inhibitors, and immune responses to vaccines. J. Virol. Methods, 2010. 169(2): p. 365-74.
- Kueck T, Bloyet LM, Cassella E, et al. Vesicular Stomatitis Virus Transcription Is Inhibited by TRIM69 in the Interferon-Induced Antiviral State. J Virol. 2019;93(24):e01372-19. Published 2019 Nov 26. View article
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