Set of Helper Plasmids (VSV-N, VSV-P, VSV-L, VSV-G)

The recovery of rVSV involves the transfection of vaccinia-T7 infected cells with 4 (or 5) different plasmids; one that expresses the rVSV antigenome (e.g. pVSV-FL+(2) or pVSV-ΔG-GFP) and 3 (or 4) other plasmids that express the VSV N, P, and L proteins (or N, P, L and G for replication-restricte ΔG-VSV).

From the laboratory of Michael A. Whitt, Ph.D., University of Tennessee.

The Investigator's Annexe Part of The Investigator's Annexe program.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
EH1012
Set of Helper Plasmids (VSV-N, VSV-P, VSV-L, VSV-G)
10uL of each (1ug/uL) In stock
Regular Price:$430.00
On Sale:
Specifications

Product Type: Plasmid
Gene/insert name: VSV nucleocapsid (N) protein; VSV phosphoprotein (P); VSV large polymerase subunit (L); VSV-Indiana glycoprotein (G)
Antibiotic Resistance: Ampicillin or Kanamycin (please see vial label and packing slip)
Fusion Tag(s): none
Concentration: 10uL (1ug/uL)
Grow in E. coli at 37 C: Yes
Vector Backbone and Size: pBS-SK-ΦT, 3105 bp
High or low copy: High
Comments: For suggested protocol, see: Whitt, MA, J. Virol. Methods, 2010. 169(2): p. 365-74.
Shipped: Ambient temperature

Provider
From the laboratory of Michael A. Whitt, Ph.D., University of Tennessee.
References
  1. Stillman, E.A., J.K. Rose, and M.A. Whitt, Replication and amplification of novel vesicular stomatitis virus minigenomes encoding viral structural proteins. J. Virol., 1995. 69: p. 2946-2953.
  2. Lawson, N.D., et al., Recombinant vesicular stomatitis viruses from DNA. Proc.Natl.Acad.Sci.(USA), 1995. 92(10): p. 4477-4481.
  3. Whitt, M.A., Generation of VSV pseudotypes using recombinant DeltaG-VSV for studies on virus entry, identification of entry inhibitors, and immune responses to vaccines. J. Virol. Methods, 2010. 169(2): p. 365-74.
  4. Kueck T, Bloyet LM, Cassella E, et al. Vesicular Stomatitis Virus Transcription Is Inhibited by TRIM69 in the Interferon-Induced Antiviral State. J Virol. 2019;93(24):e01372-19. Published 2019 Nov 26. View article

If you publish research with this product, please let us know so we can cite your paper.

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