These BHK-21 cells are highly transfectable and are used to recover infectious recombinant vesicular stomatitis virus (rVSV) as originally described by Lawson et al (5) and modified by Jayakar et al (2), or for the recovery and amplification of replication-restricted ΔG-VSV as described by Whitt (7).
The cells have been characterized by isoenzyme analysis which confirmed that the cells are from Syrian golden hamsters. The cells also have been shown to be mycoplasma-free using the Strategene Mycoplasma Plus PCR Primer Set.
From the laboratory of Michael A. Whitt, Ph.D., University of Tennessee.
Part of The Investigator's Annexe program.
|Product Type:||Cell Line|
|Cell Type:||Baby Hamster Kidney|
|Organism:||Mesocricetus auratus(hamster, Syrian golden)|
|Growth Conditions:||DMEM + 5% FBS; 37oC, 6-8% CO2|
|Subculturing:||Passage 1:5-1:3 every day, or 1:20 every 2-3 days.|
|Cryopreservation:||70% DMEM; 20% FBS; 10% DMSO|
|Comments:||For suggested protocol, see: Whitt, MA, J. Virol. Methods, 2010. 169(2): p. 365-74.|
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