pBS-G-ФT Plasmid

The plasmid pBS-G-ΦT encodes the vesicular stomatitis virus (VSV) glycoprotein (G) from the Indiana serotype expressed from the bacteriophage T7 promoter in pBS, which has been further modified to contain a T7 terminator sequence cloned between the SacII and SacI restriction sites in pBS-SK+ [1]. The plasmid is used as part of the VSV reverse genetics system to recover replication-restricted ΔG-VSV from plasmids [2]. The recovery of ΔG-VSV involves the transfection of cells that have been infected with recombinant vaccinia expressing bacteriophage T7 RNA polymerase (vvT7) with 5 different plasmids; one which expresses the ΔG-VSV antigenomic-sense (or positive-sense) RNA and 4 other plasmids that encode individually the VSV N, P, G and L proteins, all of which are under control of T7 promoters.

From the laboratory of Michael A. Whitt, Ph.D., University of Tennessee.

The Investigator's Annexe Part of The Investigator's Annexe program.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
EH1016
pBS-G-ФT Plasmid
10uL (1ug/uL) In stock
Regular Price:$115.00
On Sale:
Specifications

Product Type: Plasmid
Gene/insert name: VSV-Indiana glycoprotein (G)
Accession ID: P03522
Antibiotic Resistance: Ampicillin or Kanamycin (please see vial label and packing slip)
Fusion Tag(s): none
Concentration: 10uL (1ug/uL)
Grow in E. coli at 37 C: Yes
Cloning Site 5': ApaI
Cloning Site 3': BamHI
Insert Size: 1,700 bp
Vector Backbone and Size: pBS-SK-ΦT, 3105 bp
High or low copy: High
Comments: For suggested protocol, see: Whitt, MA, J. Virol. Methods, 2010. 169(2): p. 365-74.
Shipped: Ambient temperature

Data

Provider
From the laboratory of Michael A. Whitt, Ph.D., University of Tennessee.
References
  1. Stillman, E.A., J.K. Rose, and M.A. Whitt, Replication and amplification of novel vesicular stomatitis virus minigenomes encoding viral structural proteins. J. Virol., 1995. 69: p. 2946-2953.
  2. Whitt, M.A., Generation of VSV pseudotypes using recombinant DeltaG-VSV for studies on virus entry, identification of entry inhibitors, and immune responses to vaccines. J. Virol. Methods, 2010. 169(2): p. 365-74.
  3. Kane M, Deiss F, Chervonsky A, Golovkina TV. A Single Locus Controls Interferon Gamma-Independent Antiretroviral Neutralizing Antibody Responses. J Virol. 2018 Jul 31;92(16). pii: e00725-18. doi: 10.1128/JVI.00725-18. Print 2018 Aug 15. View Article

If you publish research with this product, please let us know so we can cite your paper.

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