Pseudotyped Δ-G/LASV-GP-NLucP rVSV

rVSVδG/LASV-GP-NLucP is a recombinant vesicular stomatitis virus in which the native glycoprotein has been replaced with the Lassa glycoprotein (GP) (Josiah strain).

The resulting virus interacts and enters cells through the Lassa GP, but once fusion has occurred, replicates using the VSV machinery. The virus also encodes nano-luciferasePEST to enable easy quantification of viral replication. Supplied virus has been passaged two times on Vero cells after the original recovery from the molecular clone.

It is the responsibility of the principal investigator to seek Institutional Biosafety Safety Committee approval for recombinant DNA, transgenic animal or infectious agent use within their laboratory spaces and maintain an Institutional Biosafety Safety Committee approval during the time period these materials are used.

From the laboratory of Melinda A. Brindley, PhD, University of Georgia.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
EGA299-PM
Pseudotyped Δ-G/LASV-GP-NLucP rVSV
0.5mL (≥ 10^6 IU/mL) In stock
Regular Price:$1,062.00
On Sale:

REQUIREMENTS

US customers - The USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to Kerafast here, in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.

Non-US customers - A BIS permit may be required in order to ship this product. Please Contact Us for more information

Specifications

Product Type: Virus
Biosafety Level: BSL2
Vector Information: Virus was generated by transfecting the molecular clone pVSV-d-G/LASV-GP-NLucP plasmid along with VSV N, P, and L into BHK-T7 cells. Virus was then amplified on Vero cells. The virus encodes nano-luciferase-PEST (NLucP) which enables viral replication to be monitored with luciferase assays. The NLucP is not secreted, so cell lysates should be monitored.
Virus: Recombinant vesicular stomatitis virus (rVSV)
Titer: 10^7 TCID50U/mL
Serotype: VSV Indiana/LASV GP Josiah strain
Inoculation Conditions: Produce stocks at low MOI (0.01) and harvest when >80% of the culture shows signs of cytopathic effect
Storage: -80C
Shipped: Dry Ice

Provider
From the laboratory of Melinda A. Brindley, PhD, University of Georgia.
References
  1. Lay Mendoza MF, Acciani MD, Levit CN, Santa Maria C, Brindley MA. Monitoring viral entry in real-time using a luciferase recombinant vesicular stomatitis virus producing SARS-CoV-2, EBOV, LASV, CHIKV, and VSV glycoproteins. Viruses. 2020 Dec 17; 12(12). PMID: 33348746

If you publish research with this product, please let us know so we can cite your paper.

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