pBS-L-ФT Plasmid

The plasmid pBS-L-ΦT encodes the large subunit (L) of the vesicular stomatitis virus (VSV) polymerase protein expressed from the bacteriophage T7 promoter in pBS, which has been further modified to contain a T7 terminator sequence cloned between the SacII and SacI restriction sites in pBS-SK+ [1]. The plasmid is used as part of the VSV reverse genetics system to recover recombinant VSV from plasmids [2, 3]. The recovery of rVSV involves the transfection of vaccinia-T7 infected cells with 4 (or 5) different plasmids; one that expresses the rVSV antigenome (e.g. pVSV-FL+(2) or pVSV-ΔG-GFP) and 3 (or 4) other plasmids that express the VSV N, P, and L proteins (or N, P, L and G for replication-restrictedΔG-VSV).

From the laboratory of Michael A. Whitt, Ph.D., University of Tennessee.

The Investigator's Annexe Part of The Investigator's Annexe program.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
EH1015
pBS-L-ФT Plasmid
10uL (1ug/uL) In stock
Regular Price:$115.00
On Sale:
Specifications

Product Type: Plasmid
Gene/insert name: VSV large polymerase subunit (L)
Accession ID: Q8B0H0
Antibiotic Resistance: Ampicillin or Kanamycin (please see vial label and packing slip)
Fusion Tag(s): none
Concentration: 10uL (1ug/uL)
Grow in E. coli at 37 C: Yes
Cloning Site 5': XhoI
Cloning Site 3': XbaI
Insert Size: 6,446 bp
Vector Backbone and Size: pBS-SK-ΦT, 3105 bp
High or low copy: High
Comments: For suggested protocol, see: Whitt, MA, J. Virol. Methods, 2010. 169(2): p. 365-74.
Shipped: Ambient temperature

Data

Provider
From the laboratory of Michael A. Whitt, Ph.D., University of Tennessee.
References
  1. Stillman, E.A., J.K. Rose, and M.A. Whitt, Replication and amplification of novel vesicular stomatitis virus minigenomes encoding viral structural proteins. J. Virol., 1995. 69: p. 2946-2953.
  2. Lawson, N.D., et al., Recombinant vesicular stomatitis viruses from DNA. Proc.Natl.Acad.Sci.(USA), 1995. 92(10): p. 4477-4481.
  3. Whitt, M.A., Generation of VSV pseudotypes using recombinant DeltaG-VSV for studies on virus entry, identification of entry inhibitors, and immune responses to vaccines. J. Virol. Methods, 2010. 169(2): p. 365-74.
  4. Lee SS, Phy K, Peden K, Sheng-Fowler L. Development of a micro-neutralization assay for ebolaviruses using a replication-competent vesicular stomatitis hybrid virus and a quantitative PCR readout. Vaccine. 2017 Apr 17. pii: S0264-410X(17)30325-0. View Article
  5. Kane M, Deiss F, Chervonsky A, Golovkina TV. A Single Locus Controls Interferon Gamma-Independent Antiretroviral Neutralizing Antibody Responses. J Virol. 2018 Jul 31;92(16). pii: e00725-18. doi: 10.1128/JVI.00725-18. Print 2018 Aug 15. View Article
  6. Rihn SJ, Aziz MA, Stewart DG, et al. TRIM69 Inhibits Vesicular Stomatitis Indiana Virus. J Virol. 2019;93(20):e00951-19. Published 2019 Sep 30. View article

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