David W. Speicher, PhD, The Wistar Institute

David W. Speicher, PhD
David W. Speicher, PhD

The Speicher laboratory is a recognized leader in the field of proteomics, the systematic study of the full set of proteins produced by a given cell, tissue, or organism. Using state-of-the-art proteomics and associated computational methods, many of which were developed or optimized in the Speicher laboratory, this research group is investigating protein changes associated with a number of different cancers, innate resistance to viral infection, red cell diseases, ectopic pregnancy and other clinical disorders. The laboratory is also using structural mass spectrometry techniques coupled with molecular modeling to determine medium resolution structures of large protein complexes and characterize conformational changes involved with physiological processes.

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References

  1. Hock RS, Davis G, Speicher DW. Purification of human smooth muscle filamin and characterization of structural domains and functional sites. Biochemistry. 1990Oct 9;29(40):9441-51.
  2. Shih IM, Speicher D, Hsu MY, Levine E, Herlyn M. Melanoma cell-cell interactions are mediated through heterophilic Mel-CAM/ligand adhesion. Cancer Res. 1997 Sep 1;57(17):3835-40.
  3. Tang HY, Speicher DW. In vivo phosphorylation of human erythrocyte spectrin occurs in a sequential manner. Biochemistry. 2004 Apr 13;43(14):4251-62.
  4. Hock RS, Davis G, Speicher DW. Purification of human smooth muscle filamin and characterization of structural domains and functional sites. Biochemistry. 1990 Oct 9;29(40):9441-51.
  5. Zimmerman UJ, Speicher DW, Fisher AB. Secretagogue-induced proteolysis of lungspectrin in alveolar epithelial type II cells. Biochim Biophys Acta. 1992 Oct27;1137(2):127-34.
  6. Speicher DW, DeSilva TM, Speicher KD, Ursitti JA, Hembach P, Weglarz L. Location of the human red cell spectrin tetramer binding site and detection of a related "closed" hairpin loop dimer using proteolytic footprinting. J Biol Chem. 1993 Feb 25;268(6):4227-35.
  7. Kotula L, DeSilva TM, Speicher DW, Curtis PJ. Functional characterization of recombinant human red cell alpha-spectrin polypeptides containing the tetramer binding site. J Biol Chem. 1993 Jul 15;268(20):14788-93.