Anti-MelCAM/MUC18, Extracellular Domain Antibody
This rabbit IgG polyclonal antibody was generated against recombinant human melanoma cell adhesion molecule (MelCAM) extracellular domain and is specific for human MelCAM/MUC18 extracellular domain.
Highlights:
- Reacts with human MelCAM/MUC18 extracellular domain
- Recommended for Western Blot applications
The human MelCAM (also known as MCAM, MUC18 or CD146) protein is a transmembrane glycoprotein, a Ca2+-independent cell adhesion molecule and is a member of a immunoglobulin superfamily. MelCAM has five immunoglobulin-like domains, a signal peptide, a transmembrane region and a cytoplasmic domain. Mel-CAM expression is only occurs is melanomas and neuroectodermal-derived cell lines. MelCAM is not expressed is normal melanocytes. Mel-CAM expression correlates with melanoma progression and matastasis. Mel-CAM activation triggers signaling by protein tyrosine kinases such as Fyn, FAK and paxillin. Mel-CAM controls the dynamics of Actin cytoskeleton rearrangement and was found to play a role in the maintenance of thymic structure and function.
From the laboratories of David W. Speicher, PhD, The Wistar Institute.
This rabbit IgG polyclonal antibody was generated against recombinant human melanoma cell adhesion molecule (MelCAM) extracellular domain and is specific for human MelCAM/MUC18 extracellular domain.
Highlights:
- Reacts with human MelCAM/MUC18 extracellular domain
- Recommended for Western Blot applications
The human MelCAM (also known as MCAM, MUC18 or CD146) protein is a transmembrane glycoprotein, a Ca2+-independent cell adhesion molecule and is a member of a immunoglobulin superfamily. MelCAM has five immunoglobulin-like domains, a signal peptide, a transmembrane region and a cytoplasmic domain. Mel-CAM expression is only occurs is melanomas and neuroectodermal-derived cell lines. MelCAM is not expressed is normal melanocytes. Mel-CAM expression correlates with melanoma progression and matastasis. Mel-CAM activation triggers signaling by protein tyrosine kinases such as Fyn, FAK and paxillin. Mel-CAM controls the dynamics of Actin cytoskeleton rearrangement and was found to play a role in the maintenance of thymic structure and function.
From the laboratories of David W. Speicher, PhD, The Wistar Institute.
| Product Type: | Antibody |
| Antigen: | Human MelCAM Extracellular Domain |
| Accession ID: | P43121 |
| Molecular Weight: | 119 kDa |
| Isotype: | IgG |
| Clonality: | Polyclonal |
| Reactivity: | Human |
| Immunogen: | Recombinant Human MelCAM Extracellular Domain |
| Species Immunized: | Rabbit |
| Purification Method: | Affinity purified rabbit serum |
| Tested Applications: | Western Blot (1:50,000; 15min exposure) |
| Concentration: | 0.586mg/mL |
| Storage: | -80C |
| Shipped: | Cold Packs (Domestic, Overnight); Dry Ice (International) |
Western Blot
Lane 1, Human Red Cell Membrane; Lane 2, Human Melanoma (1205 LU); Lane 3, Human Ovarian Cancer (RMG1); Lane 4, Human Ovarian Cancer (OVCAR3). Gel loads: Lane 1, 5mg; Lanes 2-4, 25mg. Antibody dilution: 1:50,000, 15min exposure.
- Shih IM, Speicher D, Hsu MY, Levine E, Herlyn M. Melanoma cell-cell interactions are mediated through heterophilic Mel-CAM/ligand adhesion. Cancer Res. 1997 Sep 1;57(17):3835-40.
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