Anti-Beta-Spectrin, C-Terminal [SP316] Antibody

This mouse IgG monoclonal antibody were generated against recombinant peptide (residues 1902-2037 fused with GST) and is specific for human red blood cell beta-spectrin isoform (alternatively spliced C-terminal), residues 2085-2137.

Highlights:

  • Reacts with human red blood cell beta-spectrin isoform (alternatively spliced C-terminal)
  • Capable of recognizing the C-terminal region of β-spectrin regardless of its phosphorylation state
  • Used it to purify the phosphorylated region after 2-nitro-5-thiocyanobenzoic acid cleavage of spectrin
  • Recommended for Western Blot applications

Spectrin is a cytoskeletal protein that lines the intracellular side of the plasma membrane in eukaryotic cells along with actin. Spectrin plays a role in cell shape control, arrangement of transmembrane proteins, and organelle positioning. Beta-Spectrin functions as a structural element and stabilizes erythrocyte membranes. Mutations in beta-spectrin are described as a possible cause of neonatal hemolytic anemia, spherocytosis type 2, and hereditary elliptocytosis.

From the laboratories of David W. Speicher, PhD, The Wistar Institute.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
EWI031
Anti-Beta-Spectrin, C-Terminal [SP316] Antibody
50uL In stock
Regular Price:$306.00
On Sale:
Specifications

Product Type: Antibody
Antigen: Human red blood cell beta-spectrin isoform (alternatively spliced C-terminal)
Accession ID: HGNC: 11274 Entrez Gene: 6710 Ensembl: ENSG00000070182 OMIM: 182870 UniProtKB: P11277
Molecular Weight: 246 kDa
Isotype: IgG
Clonality: Monoclonal
Clone Name: SP316
Reactivity: Human
Immunogen: Recombinant peptide (residues 1902-2037 fused with GST)
Species Immunized: Mouse
Epitope: Residues 2085-2137
Purification Method: Affinity purified
Tested Applications: Western Blot (1:250,000; 20sec exposure)
Storage: -20C
Shipped: Cold packs

Data

Western Blot

Lane 1, Human Melanoma (1205 LU); Lane 2, Human Ovarian Cancer (RMG1); Lane 3, Human Ovarian Cancer (OV90); Lane 4, Human Red Cell Membrane. Gel loads: Lanes 1-3, 25mg; Lane 4, 5mg. Antibody dilution: 1:250,000, 20sec exposure.

Provider
From the laboratories of David W. Speicher, PhD, The Wistar Institute.
References
  1. Tang HY, Speicher DW. In vivo phosphorylation of human erythrocyte spectrin occurs in a sequential manner. Biochemistry. 2004 Apr 13;43(14):4251-62.

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