This mouse IgG monoclonal antibody were generated against recombinant peptide (residues 1902-2037 fused with GST) and is specific for human red blood cell beta-spectrin isoform (alternatively spliced C-terminal), residues 2085-2137.
Spectrin is a cytoskeletal protein that lines the intracellular side of the plasma membrane in eukaryotic cells along with actin. Spectrin plays a role in cell shape control, arrangement of transmembrane proteins, and organelle positioning. Beta-Spectrin functions as a structural element and stabilizes erythrocyte membranes. Mutations in beta-spectrin are described as a possible cause of neonatal hemolytic anemia, spherocytosis type 2, and hereditary elliptocytosis.
From the laboratories of David W. Speicher, PhD, The Wistar Institute.
|Antigen:||Human red blood cell beta-spectrin isoform (alternatively spliced C-terminal)|
|Molecular Weight:||246 kDa|
|Immunogen:||Recombinant peptide (residues 1902-2037 fused with GST)|
|Purification Method:||Affinity purified|
|Tested Applications:||Western Blot (1:250,000; 20sec exposure)|
Lane 1, Human Melanoma (1205 LU); Lane 2, Human Ovarian Cancer (RMG1); Lane 3, Human Ovarian Cancer (OV90); Lane 4, Human Red Cell Membrane. Gel loads: Lanes 1-3, 25mg; Lane 4, 5mg. Antibody dilution: 1:250,000, 20sec exposure.
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