This rabbit IgG polyclonal antibody was generated against smooth muscle actin and filamin and is specific for human actin, beta-actin.
Beta-actin is a highly conserved protein that are involved in cell motility, structure, and integrity. Beta- actin, is one of the six isoforms in humans, and one of the two nonmuscle cytoskeletal actins. Beta-actin is commonly used as a loading control.
From the laboratories of David W. Speicher, PhD, The Wistar Institute.
|Antigen:||Human Actin, beta-actin|
|Accession ID:||HGNC: 132 Entrez Gene: 60 Ensembl: ENSG00000075624 OMIM: 102630 UniProtKB: P60709|
|Molecular Weight:||42 kDa|
|Immunogen:||Smooth muscle actin and filamin|
|Purification Method:||Rabbit serum was affinity purified to separate only the antibody that binds to actin only|
|Tested Applications:||Western Blot (1:2,000; 1min exposure)|
Lane 1, Human Red Cell Membrane; Lane 2, Human Melanoma (1205 LU); Lane 3, Human Ovarian Cancer (RMG1); Lane 4, Human Ovarian Cancer (OVCAR3); Lane 5, Human Ovarian Cancer (OV90). Gel loads: Lane 1, 5mg; Lanes 2-5, 25mg. Antibody dilution: 1:2,000, 1min exposure.
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