Anti-Beta-Actin Antibody

This rabbit IgG polyclonal antibody was generated against smooth muscle actin and filamin and is specific for human actin, beta-actin.

Highlights:

  • Reacts with human actin, beta-actin
  • Recommended for Western Blot applications

Beta-actin is a highly conserved protein that are involved in cell motility, structure, and integrity. Beta- actin, is one of the six isoforms in humans, and one of the two nonmuscle cytoskeletal actins. Beta-actin is commonly used as a loading control.

From the laboratories of David W. Speicher, PhD, The Wistar Institute.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
EWI032
Anti-Beta-Actin Antibody
100uL In stock
Regular Price:$306.00
On Sale:
Specifications

Product Type: Antibody
Antigen: Human Actin, beta-actin
Accession ID: HGNC: 132 Entrez Gene: 60 Ensembl: ENSG00000075624 OMIM: 102630 UniProtKB: P60709
Molecular Weight: 42 kDa
Isotype: IgG
Clonality: Polyclonal
Reactivity: Human
Immunogen: Smooth muscle actin and filamin
Species Immunized: Rabbit
Purification Method: Rabbit serum was affinity purified to separate only the antibody that binds to actin only
Tested Applications: Western Blot (1:2,000; 1min exposure)
Storage: -80C
Shipped: Dry ice

Data

Western Blot

Lane 1, Human Red Cell Membrane; Lane 2, Human Melanoma (1205 LU); Lane 3, Human Ovarian Cancer (RMG1); Lane 4, Human Ovarian Cancer (OVCAR3); Lane 5, Human Ovarian Cancer (OV90). Gel loads: Lane 1, 5mg; Lanes 2-5, 25mg. Antibody dilution: 1:2,000, 1min exposure.

Provider
From the laboratories of David W. Speicher, PhD, The Wistar Institute.
References
  1. Hock RS, Davis G, Speicher DW. Purification of human smooth muscle filamin and characterization of structural domains and functional sites. Biochemistry. 1990 Oct 9;29(40):9441-51.

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