This rabbit IgG polyclonal antibody was generated against human red blood cell a-spectrin N terminal 1/4th and is specific for human red blood cell a-spectrin N terminal 1/4th of the protein.
Spectrin is a cytoskeletal protein that lines the intracellular side of the plasma membrane in eukaryotic cells along with actin. Spectrin plays a role in cell shape control, arrangement of transmembrane proteins, and organelle positioning. Specifically, Alpha-spectrin is a tetramer and mutations in alpha-spectrin can result in a variety of hereditary red blood cell disorders (e.g. spherocytic hemolytic anemia including elliptocytosis type 2, pyropoikilocytosis).
From the laboratories of David W. Speicher, PhD, The Wistar Institute.
|Alternative Name(s):||Erythroid alpha-spectrin,Spectrin alpha chain, erythrocytic 1|
|Antigen:||Human Red Blood Cell a-Spectrin N terminal 1/4th of the protein|
|Molecular Weight:||280 kDa|
|Immunogen:||Alpha-spectrin 80kDa domain (approximately residues 7-601)|
|Tested Applications:||Western Blot (1: 500,000; 5sec exposure)|
Lane 1, Human Red Cell Membrane; Lane 2, Human Melanoma (1205 LU); Lane 3, Human Ovarian Cancer (RMG1); Lane 4, Human Ovarian Cancer (OV90). Gel loads: Lane 1, 5mg; Lanes 2-4, 25mg. Antibody dilution: 1:500,000, 5sec exposure.
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