Lynda F. Bonewald, PhD, University of Missouri - Kansas City

Lynda F. Bonewald, PhD
Lynda F. Bonewald, PhD

Dr. Bonewald's research has focused on osteocyte biology since and has develeoped recently, a cell line that replicates late osteoblast to late osteocytes, IDG-SW3 (JBMR 2011). Osteocytes are the most abundant bone cell in the adult skeleton. In the last decade, it has been determined that these cells are multifunctional including 1). Acting as orchestrators to regulate bone remodeling through direct actions on both osteoblasts and osteoclasts, 2). As endocrine cells regulating distant organs such as kidney and muscle, 3). As mechanosensory cells determining bone response to loading and unloading, 4). As regulators of the mineralization process, and 5). Regulators of mineral homeostasis. At present she is studying osteoblast to osteocyte differentiation, the functions of the osteocyte during lactation, and the crosstalk between osteocytes and muscle.

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References:

  1. Zhao N, Nociti Jr FH, Duan P, Prideaux M, Zhao H, Foster BL, Somerman MJ, Bonewald LF. Isolation and Functional Analysis of an Immortalized Murine Cementocyte Cell Line, IDG-CM6. J Bone Miner Res. 2016 Feb: 31(2): 430-442. DOI: 10.1002/jbmr.2690
  2. Zhao N, Foster BL, Bonewald LF. The Cementocyte-An Osteocyte Relative? J Dental Res. 2016, Vol 95 (7) 734-741. DOI: 10.1177/0022034516641898
  3. St John HC, Bishop KA, Meyer MB, Benkusky NA, Leng N, Kendziorski C, Bonewald LF, Pike JW. The osteoblast to osteocyte transition: epigenetic changes and response to the vitamin d3 hormone. Mol Endocrinol. 2014 Jul;28(7):1150-65.
  4. Fujita K, Xing Q, Khosla S, Monroe DG. Mutual Enhancement of Differentiation of Osteoblasts and Osteocytes Occurs Through Direct Cell-Cell Contact. J Cell Biochem. 2014 Jul 15.
  5. Ito N, Findlay DM, Anderson PH, Bonewald LF, Atkins GJ. Extracellular phosphate modulates the effect of 1α,25-dihydroxy vitamin D3 (1,25D) on osteocyte like cells. J Steroid Biochem Mol Biol. 2013 Jul;136:183-6.
  6. Rosser J, Bonewald LF. Studying osteocyte function using the cell lines MLO-Y4 and MLO-A5. Methods Mol Biol. 2012;816:67-81.
  7. Woo SM, Rosser J, Dusevich V, Kalajzic I, Bonewald LF. Cell line IDG-SW3 replicates osteoblast-to-late-osteocyte differentiation in vitro and accelerates bone formation in vivo. J Bone Miner Res. 2011 Nov;26(11):2634-46.
  8. Kato, Y, Boskey, A, Spevak, L, Dallas, M, Hori, M, Bonewald, LF, Establishment of an Osteoid Pre-Osteocyte like cell, MLO-A5, that spontaneously mineralizes in culture without the addition of beta-glycerol phosphate and ascorbic acid. J. Bone Min. Res. 16:1622-1633, 2001.
  9. Kato Y, Windle J,Koop B, Qiao M, Bonewald L. Establishment of an osteocyte-like cell line, MLO-Y4 J Bone Min. Res. 12:2014-2023, 1997.