Cell line IDG-SW3 replicates osteoblast to late osteocyte differentiation in vitro and increases bone formation in vivo.
Osteocytes are the most abundant bone cells in the body but also the most challenging to study because they are embedded in a mineralized matrix making them to difficult to isolate. This cell line should be useful study osteoblast to osteocyte transition, mechanisms for biomineralization osteocyte function and regulation of SOST/sclerostin and FGF-23.
From the laboratory of Lynda F. Bonewald, PhD, University of Missouri - Kansas City.
|Product Type:||Cell Line|
|Cell Type:||Osteoblast/early osteocyte/late osteocyte|
|Morphology:||Adherent osteoblast-like cell|
|Subculturing:||See: IDG-SW3 Cell Line Protocol|
Proliferation medium: AlphaMEM (containing L-glutamine and deoxyribonucleosides); supplemented with 10% FBS; 1% penicillin-streptomycin; Recombinant Mouse Interferon-gamma (INF-γ) 50 U/ml. Grown on dishes coated with [0.15 mg/ml] rat tail type I collagen. Incubate at 33°C with 5% CO2
Differentiation medium: AlphaMEM (L-glutamine and deoxyribonucleosides); supplemented with 10% FBS; 1% penicillin-streptomycin; 50µg/ml Ascorbic Acid and 4mM β-glycerophosphate. Grown on dishes coated with [0.15 mg/ml] rat tail type I collagen. Incubate at 37°C with 8% CO2.
|Cryopreservation:||60% AlphaMEM, 30% FBS, 10% DMSO, at 1-2 x 10^6 cells/vial/1ml|
Osteoblastic and osteocytic markers
Schematic diagram summarizing ostoblastic and osteocytic markers in IDG-SW3 cells over time. IDG-SW3 cells transition from late osteoblasts to late osteocytes in vitro on both 2D and 3D substrates.
Adapted from: Woo SM. et al. J Bone Miner Res. 2011 Nov;26(11):2634-46.
NOTE: Mineralization and gene expression may be Fetal Bovine Serum dependent; testing and optimization of different serum lots/batches may be necessary. Mineralization and gene expression may be CO2 dependent; testing and optimization of 5-10% CO2 may be necessary. Testing with our current serum showed that differentiation of the IDG-SW3 in 8% CO2 resulted in an increase of mineral, and in SOST and DMP1 gene expression, when compared to cells differentiated in 5% CO2.
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