MLO-Y4 cell line is useful to study effects of fluid flow and substrate stretching on activation of osteocyte-specific signaling pathways.
Osteocytes are the most abundant bone cells in the body but also the most challenging to study because they are embedded in a mineralized matrix making them to difficult to isolate. This cell line MLO-Y4 makes it easier to study osteocyte function.
From the laboratory of Lynda Bonewald, PhD, University of Missouri - Kansas City.
Part of The Investigator's Annexe program.
|Product Type:||Cell Line|
|Subculturing:||Maintain stock cultures in complete culture medium at ~60-75% confluence on collagen coated plates at 37°C and at 5% CO 2. Passage at ~ 1:5 dilution (~1:7maximum) using 0.05% Trypsin/0.53 mM EDTA every 3-4 days (see Rosser J. et al. Methods Mol Biol. 2012;816:67-81|
|Growth Conditions:||alpha-MEM, containing L-glutamine and deoxyribonucleosides, with penicillin/streptomycin at 100 U/ml and 2.5% FBS and 2.5% CS; both heat inactivated; Grown on dishes coated with [0.15 mg/ml] rat tail type 1 collagen (see Rosser J. et al. Methods Mol Biol. 2012;816:67-81).|
|Cryopreservation:||60% alpha-MEM, 30% FBS, 10% DMSO, at 1 × 10 6cells/ml/cryovial (see Rosser J. et al. Methods Mol Biol. 2012;816:67-81)|
|Comments:||When first starting these cells, centrifuge at approx. 1,000 rpm, for 5–10 min. Aspirate media, gently resuspend and plate the cell pellet in media containing 5% FBS and 5% CS. This higher amount of serum, 10% total, is useful to give the cells an extra boost. The next day, check the viability of the cells. If there are a lot of fl oating “dead” cells, change the medium. You can revert back to medium with 2.5% FBS + 2.5% CS at this point.|
Appearance of the MLO-Y4 cells at 4 and 12 h after plating. The cells are attached by 4 h and already generate dendritic processes by 12 h.
Adapted from: Rosser J. et al. Methods Mol Biol. 2012;816:67-81.
If you publish research with this product, please let us know so we can cite your paper.