MLO-A5 cell line is a model for the osteoblast to osteocyte differentiation process, the mineralization process, and the effects of mechanical loading on biomineralization.
Osteocytes are the most abundant bone cells in the body but also the most challenging to study because they are embedded in a mineralized matrix making them to difficult to isolate. This cell line MLO-A5 make it easier to study osteocyte function.
From the laboratory of Lynda Bonewald, PhD, University of Missouri - Kansas City.
|Product Type:||Cell Line|
|Morphology:||Adherent osteoblast-like cell; honeycomb-like mineralized matrix within 7-9 days of culture|
Proliferation medium: AlphaMEM (containing L-glutamine and deoxyribonucleosides); supplemented with 5% FBS and 5% CS, both heat-inactivated; penicillin-streptomycin at 100U/ml-100ug/ml
Differentiation medium: AlphaMEM (L-glutamine and deoxyribonucleosides); supplemented with 10% FBS; penicillin-streptomycin at 100U/ml-100ug/ml; approximately 100µg/ml Ascorbic Acid and 4mM β-glycerophosphate (see Comments).
Grown on dishes coated with [0.15 mg/ml] rat tail type I collagen.
|Subculturing:||Maintain stock cultures in proliferation medium under subconfluent conditions on collagen coated plates at 37°C and at 5% CO 2. Passage at ~ 1:10 to 1:20 dilution using 0.05% Trypsin/0.53 mM EDTA every 3-4 days (see Rosser J. et al. Methods Mol Biol. 2012;816:67-81)|
|Cryopreservation:||60% alpha-MEM, 30% FBS, 10% DMSO, at 1 × 10 6 cells/ml/cryovial (see Rosser J. et al. Methods Mol Biol. 2012;816:67-81)|
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