Anti-LacI [17D5] Antibody

This IgG mouse monoclonal (17D5) was generated against recombinant LacI+NLS+6His and is reactive against E. coli LacI.

Highlights:

  • Reacts with native or a recombinant version of LacI expressed in E. coli or Arabidopsis
  • Suitable for Western Blot

Lac repressor (LacI) is a DNA-binding protein which inhibits the expression of genes coding for proteins involved in the metabolism of lactose in bacteria. In the absence of lactose, the Lac repressor binds to the operator and keeps RNA polymerase from transcribing the Lac genes.

Also available: Anti-LacI [15C11] Antibody.

From the laboratory of Richard B. Meagher, PhD, University of Georgia

The Investigator's Annexe Part of The Investigator's Annexe program.

Catalog Number Product Size AVAILABILITY Price Qty
EGA011
Anti-LacI [17D5] Antibody
100ug 2-3 weeks
Regular Price:$350.00
Specifications

Product Type: Antibody
Antigen: LacI
Accession ID: AY042185
Molecular Weight: 45 kDa, 375 amino acids
Isotype: IgG1
Clonality: Monoclonal
Clone Name: 17D5
Reactivity: E. coliand ArabidopsisLacI
Immunogen: Recombinant LacI-NLS-His6 made in E. coli
Species Immunized: Mouse
Buffer: 0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide
Tested Applications: WB (5ug in 10mL)
Storage: -20C
Shipped: Cold packs

Data

Western Blot

Reactivity is detected in a crude protein extract (2 ug) of E. coli expressing recombinant LacI+NLS+His6 protein, crude extracts (20 ug) from two stains of E. coli (MC1693, SK1029) expressing WT LacI at different levels, and extracts (30 ug) from three independent Arabidopsis thaliana (At) plant lines (#1, #2, #3) expressing recombinant LacI+NLS+His6. No reactivity is detected in the E. coli LacI deficient strain MC1061.

Adapted from: Kim T., et al., Plant Biotechnol J. 2005 Nov;3(6):571-82.

Provider
From the laboratory of Richard B. Meagher, PhD, University of Georgia
References
  1. Kim T, Balish RS, Heaton AC, McKinney EC, Dhankher OP, Meagher RB. Engineering a root-specific, repressor-operator gene complex. Plant Biotechnol J. 2005 Nov;3(6):571-82.

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