Anti-LacI [15C11] Antibody
![Anti-LacI [15C11] Antibody](https://www.kerafast.com/MediaStorage/Product/Images/Large/879_200120200126258780.jpg "Anti-LacI [15C11] Antibody")
This IgG mouse monoclonal (15C11) was generated against recombinant LacI+NLS+6His and is reactive against E. coli and Arabidopsis LacI.
Highlights:
- Reacts with native and recombinant LacI expressed in E. coli or Arabidopsis
- Suitable for Western Blot
Lac repressor (LacI) is a DNA-binding protein which inhibits the expression of genes coding for proteins involved in the metabolism of lactose in bacteria. In the absence of lactose, the Lac repressor binds to the operator and keeps RNA polymerase from transcribing the Lac genes.
Also available: Anti-LacI [17D5] Antibody.
From the laboratory of Richard B. Meagher, PhD, University of Georgia.
Part of The Investigator's Annexe program.
This IgG mouse monoclonal (15C11) was generated against recombinant LacI+NLS+6His and is reactive against E. coli and Arabidopsis LacI.
Highlights:
- Reacts with native and recombinant LacI expressed in E. coli or Arabidopsis
- Suitable for Western Blot
Lac repressor (LacI) is a DNA-binding protein which inhibits the expression of genes coding for proteins involved in the metabolism of lactose in bacteria. In the absence of lactose, the Lac repressor binds to the operator and keeps RNA polymerase from transcribing the Lac genes.
Also available: Anti-LacI [17D5] Antibody.
From the laboratory of Richard B. Meagher, PhD, University of Georgia.
Part of The Investigator's Annexe program.
| Product Type: | Antibody |
| Antigen: | LacI |
| Accession ID: | P03023 , AY042185 |
| Molecular Weight: | 45 kDa, 375 amino acids |
| Isotype: | IgG1 |
| Clonality: | Monoclonal |
| Clone Name: | 15C11 |
| Reactivity: | E. coliand ArabidopsisLacI |
| Immunogen: | Recombinant LacI-NLS-His6 made in E. coli |
| Species Immunized: | Mouse |
| Buffer: | PBS, 0.05% (w/v) Sodium Azide |
| Tested Applications: | WB (5ug in 10mL) |
| Storage: | -20C |
| Shipped: | Cold packs |
Western Blot
Reactivity is detected in a crude protein extract (2 ug) of E. coli expressing recombinant LacI+NLS+His6 protein, crude extracts (20 ug) from two stains of E. coli (MC1693, SK1029) expressing WT LacI at different levels, and extracts (30 ug) from three independent Arabidopsis thaliana (At) plant lines (#1, #2, #3) expressing recombinant LacI+NLS+His6. No reactivity is detected in the E. coli LacI deficient strain MC1061. Both mAbs are IgG1 isotypes.
Adapted from: Kim T., et al., Plant Biotechnol J. 2005 Nov;3(6):571-82.
- Kim T, Balish RS, Heaton AC, McKinney EC, Dhankher OP, Meagher RB. Engineering a root-specific, repressor-operator gene complex. Plant Biotechnol J. 2005 Nov;3(6):571-82.
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