Dominic Esposito, PhD, National Cancer Institute/NIH

Dominic Esposito, PhD
Dominic Esposito, PhD

Dr. Esposito is the director of the Protein Expression Laboratory (PEL) at the Frederick National Laboratory for Cancer Research in Frederick, MD. The PEL produces proteins and other reagents for the NCI RAS Initiative as well as for NCI, NIH, and other governmental organizations, and develops new technologies for protein expression and production.



  1. Mehalko JL, Esposito D. Engineering the transposition-based baculovirus expression vector system for higher efficiency protein production from insect cells. J Biotechnol. 2016 Nov 20;238:1-8.
  2. Gillette WK, Esposito D, Abreu Blanco M, Alexander P, Bindu L, Bittner C, Chertov O, Frank PH, Grose C, Jones JE, Meng Z, Perkins S, Van Q, Ghirlando R, Fivash M, Nissley DV, McCormick F, Holderfield M, Stephen AG. Farnesylated and methylated KRAS4b: high yield production of protein suitable for biophysical studies of prenylated protein-lipid interactions. Sci Rep. 2015 Nov 2;5:15916. doi: 10.1038/srep15916. PubMed PMID: 26522388; PubMed Central PMCID: PMC4629113.
  3. Gillette WK, Esposito D, Taylor TE, Hopkins RF, Bagni RK, Hartley JL. Purify First: rapid expression and purification of proteins from XMRV. Protein Expr Purif. 2011 Apr;76(2):238-47.
  4. Hopkins R, Esposito D. A rapid method for titrating baculovirus stocks using the Sf-9 Easy Titer cell line. Biotechniques. 2009 Sep;47(3):785-8.
  5. Day CP, Carter J, Bonomi C, Esposito D, Crise B, Ortiz-Conde B, Hollingshead M, Merlino G. Lentivirus-mediated bifunctional cell labeling for in vivo melanoma study. Pigment Cell Melanoma Res. 2009 Jun;22(3):283-95.