These plasmids are pFastBac style baculovirus expression vectors in which human KRAS is expressed from the baculovirus polyhedrin promoter with a maltose binding protein solubility tag.
KRAS gene makes a protein called K-Ras that is involved primarily in regulating cell division. As part of a signaling pathway known as the RAS/MAPK pathway, the protein relays signals from outside the cell to the cell's nucleus. These signals instruct the cell to grow and divide or to mature and take on specialized functions (differentiate). The KRAS gene is also an oncogene, when mutated normal cells can be become cancerous.
From the laboratory of Dominic Esposito, PhD, National Cancer Institute/NIH.
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|Name:||KRAS4b Baculovirus Expression Vectors|
|Gene/insert name:||Human KRAS4b|
|Fusion Tag(s):||Aminoterminal His6-MBP-tev|
|Format:||Liquid (10 mM Tris-Cl, pH 8.0, 0.1 mM EDTA)|
|Grow in E. coli at 37 C:||Yes|
|Cloning Site 5':||Gateway attB1|
|Cloning Site 3':||Gateway attB2|
|Insert Size:||570 bp|
|Vector Backbone and Size:||pDest-636, 6050 bp|
|High or low copy:||High|
|Comments:||To create baculovirus, must be transformed into a DH10Bac or DE35 type strain|
|Shipped:||Room Temperature as a liquid|
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