Trichoplusia ni cell line derived from BTI-Tn-5B1-4, adapted for suspension growth and serum-free growth.
The baculovirus expression vector system (BEVS) has proven to be a powerful technology for expression of recombinant proteins in eukaryotic cells. The BEVS technology has many advantages that include the capacity for large DNA insertions, the capacity for simultaneous expression of multiple genes, high expression levels for many proteins, and posttranslational modifications that often resemble those found in mammalian cells.
From the laboratory of Dominic Esposito, PhD, National Cancer Institute/NIH.
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|Product Type:||Cell Line|
|Subculturing:||5 x 105 cells/ml for 4 day growth, 6 x 105 cells/ml for 3 day growth|
Sf-900 III Serum Free Media; 21 hour doubling time, Max Growth 5 x 106 cells/ml. Temperature: 27C. Growth Conditions: Shake culture at 125 RPM for shaker board with 2" orbit or 185 RPM with a 1" orbit in Thomson Instrument Optimum Growth Flasks.
|Cryopreservation:||90% Growth Media; 10% DMSO; 0.15M Trehalose|
|Comments:||Use of 10 units/ml of Heparin can be used on highly aggregated cultures to disperse aggregation|
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