Timothy J. Stasevich, PhD, Colorado State University

Timothy J. Stasevich, PhD
Timothy J. Stasevich, PhD

The Stasevich laboratory has developed fluorescence microscopy tools to image proteins in living cells that are difficult if not impossible to see using standard techniques. They are using these techniques to answer long-standing questions about how epigenetic factors contribute to gene misregulation in human disease. Currently they are focusing on two hard-to-see protein populations: (1) nascent proteins in the process of being translated (while still attached to RNA) and (2) post-translationally modified proteins, particularly modified chromatin and modified transcription machinery.

 

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References

  1. Zhao N, Kamijo K, Fox PD, Oda H, Morisaki T, Sato Y, Kimura H, Stasevich TJ. A genetically encoded probe for imaging nascent and mature HA-tagged proteins in vivo. Nat Commun. 2019 Jul 3;10(1):2947. doi: 10.1038/s41467-019-10846-1. PMID: 31270320; PMCID: PMC6610143.
  2. Liu Y, Zhao N, Kanemaki MT, Yamamoto Y, Sadamura Y, Ito Y, Tokunaga M, Stasevich TJ, Kimura H. Visualizing looping of two endogenous genomic loci using synthetic zinc-finger proteins with anti-FLAG and anti-HA frankenbodies in living cells. Genes Cells. 2021 Nov;26(11):905-926. doi: 10.1111/gtc.12893. Epub 2021 Sep 20. PMID: 34465007; PMCID: PMC8893316.
  3. Cialek CA, Galindo G, Morisaki T, Zhao N, Montgomery TA, Stasevich TJ. Imaging translational control by Argonaute with single-molecule resolution in live cells. Nat Commun. 2022 Jun 10;13(1):3345. doi: 10.1038/s41467-022-30976-3. PMID: 35688806; PMCID: PMC9187665.