HA Frankenbody Expression Plasmids

Plasmids encoding anti-HA single-chain variable fragments (scFv) fused with mEGFP, HaloTag, or mCherry under CMV promoter.

Highlights:

  • Encode mouse anti-HA- scFvs for H. sapiens (human) expression
  • Engineered scFv binding the linear HA epitope with high affinity and specificity in vivo
  • Light up in multiple colors HA-tagged nuclear, cytoplasmic, membrane, and mitochondrial proteins in diverse cell types
  • Precisely quantify the expression of HA-tagged proteins in live cells
  • Powerful tool for imaging protein dynamics in vivo

These plasmids genetically encode scFvs that selectively bind the HA epitope (YPYDVPDYA) with high affinity in living cells and organisms and can be used to quantify HA-tagged protein translation, localization, and dynamics. The plasmids enable, state-of-the-art, single-molecule experiments in living cells, which we demonstrate by tracking single HA-tagged histones in U2OS cells and single mRNA translation dynamics in both U2OS cells and neurons.

From the laboratory of Timothy J. Stasevich, PhD, Colorado State University.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
ECS015
pNZ035, Anti-HA scFv-mEGFP Plasmid
250ng In stock
Regular Price:$66.00
On Sale:
ECS016
pNZ055, Anti-HA scFv-HaloTag Plasmid
250ng In stock
Regular Price:$66.00
On Sale:
ECS017
pNZ053, Anti-HA scFv-mCherry Plasmid
250ng In stock
Regular Price:$66.00
On Sale:
Specifications

Product Type: Plasmid
Antibiotic Resistance: Kanamycin
Fusion Tag(s): mEGFP, HaloTag, or mCherry
Tested Applications: Resulting antibodies can be used for intracellular studies
Grow in E. coli at 37 C: Yes
Selectable markers: Kanamycin
Cloning Site 5': EcoRI
Cloning Site 3': NotI
Insert Size: 1584 bp (pNZ035), 1758bp (pNZ055), 1578bp (pNZ053)
Vector Backbone and Size: pmCherry-N1
High or low copy: High
Promoter: CMV driven
Storage: -20C
Shipped: Room temperature

Provider
From the laboratory of Timothy J. Stasevich, PhD, Colorado State University.
References
  1. Zhao N, Kamijo K, Fox PD, Oda H, Morisaki T, Sato Y, Kimura H, Stasevich TJ. A genetically encoded probe for imaging nascent and mature HA-tagged proteins in vivo. Nat Commun. 2019 Jul 3;10(1):2947. doi: 10.1038/s41467-019-10846-1. PMID: 31270320; PMCID: PMC6610143.

If you publish research with this product, please let us know so we can cite your paper.

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