HA Frankenbody Expression Plasmids
Plasmids encoding anti-HA single-chain variable fragments (scFv) fused with mEGFP, HaloTag, or mCherry under CMV promoter.
Highlights:
- Encode mouse anti-HA- scFvs for H. sapiens (human) expression
- Engineered scFv binding the linear HA epitope with high affinity and specificity in vivo
- Light up in multiple colors HA-tagged nuclear, cytoplasmic, membrane, and mitochondrial proteins in diverse cell types
- Precisely quantify the expression of HA-tagged proteins in live cells
- Powerful tool for imaging protein dynamics in vivo
These plasmids genetically encode scFvs that selectively bind the HA epitope (YPYDVPDYA) with high affinity in living cells and organisms and can be used to quantify HA-tagged protein translation, localization, and dynamics. The plasmids enable, state-of-the-art, single-molecule experiments in living cells, which we demonstrate by tracking single HA-tagged histones in U2OS cells and single mRNA translation dynamics in both U2OS cells and neurons.
From the laboratory of Timothy J. Stasevich, PhD, Colorado State University.
Plasmids encoding anti-HA single-chain variable fragments (scFv) fused with mEGFP, HaloTag, or mCherry under CMV promoter.
Highlights:
- Encode mouse anti-HA- scFvs for H. sapiens (human) expression
- Engineered scFv binding the linear HA epitope with high affinity and specificity in vivo
- Light up in multiple colors HA-tagged nuclear, cytoplasmic, membrane, and mitochondrial proteins in diverse cell types
- Precisely quantify the expression of HA-tagged proteins in live cells
- Powerful tool for imaging protein dynamics in vivo
These plasmids genetically encode scFvs that selectively bind the HA epitope (YPYDVPDYA) with high affinity in living cells and organisms and can be used to quantify HA-tagged protein translation, localization, and dynamics. The plasmids enable, state-of-the-art, single-molecule experiments in living cells, which we demonstrate by tracking single HA-tagged histones in U2OS cells and single mRNA translation dynamics in both U2OS cells and neurons.
From the laboratory of Timothy J. Stasevich, PhD, Colorado State University.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
|---|
Specifications
| Product Type: | Plasmid |
| Antibiotic Resistance: | Kanamycin |
| Fusion Tag(s): | mEGFP, HaloTag, or mCherry |
| Tested Applications: | Resulting antibodies can be used for intracellular studies |
| Grow in E. coli at 37 C: | Yes |
| Selectable markers: | Kanamycin |
| Cloning Site 5': | EcoRI |
| Cloning Site 3': | NotI |
| Insert Size: | 1584 bp (pNZ035), 1758bp (pNZ055), 1578bp (pNZ053) |
| Vector Backbone and Size: | pmCherry-N1 |
| High or low copy: | High |
| Promoter: | CMV driven |
| Storage: | -20C |
| Shipped: | Room temperature |
Provider
From the laboratory of Timothy J. Stasevich, PhD, Colorado State University.
References
- Zhao N, Kamijo K, Fox PD, Oda H, Morisaki T, Sato Y, Kimura H, Stasevich TJ. A genetically encoded probe for imaging nascent and mature HA-tagged proteins in vivo. Nat Commun. 2019 Jul 3;10(1):2947. doi: 10.1038/s41467-019-10846-1. PMID: 31270320; PMCID: PMC6610143.
- Cialek CA, Galindo G, Morisaki T, Zhao N, Montgomery TA, Stasevich TJ. Imaging translational control by Argonaute with single-molecule resolution in live cells. Nat Commun. 2022 Jun 10;13(1):3345. doi: 10.1038/s41467-022-30976-3. PMID: 35688806; PMCID: PMC9187665.
If you publish research with this product, please let us know so we can cite your paper.
