FLAG-Frankenbody Expression Plasmids

Plasmids encoding anti-FLAG single-chain variable fragment (scFv) fused with mEGFP or HaloTag under CMV promoter.

Highlights:

  • Encode mouse anti-FLAG- scFvs for H. sapiens (human) expression
  • Engineered scFv binding the linear FLAG epitope with high affinity and specificity in vivo
  • Light up in multiple colors FLAG-tagged nuclear, cytoplasmic, membrane, and mitochondrial proteins in diverse cell types
  • Precisely quantify the expression of FLAG-tagged proteins in live cells
  • Powerful tool for imaging protein dynamics in vivo

These plasmids genetically encode intracellular single-chain variable fragment that selectively binds the FLAG epitope (DYKDDDDK) with high affinity in living cells and organisms and can be used to quantify FLAG-tagged protein translation, localization, and dynamics.

From the laboratory of Timothy J. Stasevich, PhD, Colorado State University.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
ECS019
Anti-FLAG scFv-HaloTag Plasmid
250ng In stock
Regular Price:$66.00
On Sale:
ECS020
Anti-FLAG scFv-GFP Plasmid
250ng In stock
Regular Price:$66.00
On Sale:
Specifications

Product Type: Plasmid
Gene/insert name: anti-FLAG-scFv
Organism: Mouse
Fusion Tag(s): mEGFP or HaloTag
Tested Applications: Resulting antibodies can be used for intracellular studies
Grow in E. coli at 37 C: Yes
Selectable markers: Kanamycin
Cloning Site 5': EcoRI
Cloning Site 3': NotI
Insert Size: 1568bp (scFv-GFP) or 1742 bp (scFv-HaloTag)
Vector Backbone and Size: pmCherry-C1
High or low copy: High
Promoter: CMV driven
Storage: -20C
Shipped: Room temperature

Provider
From the laboratory of Timothy J. Stasevich, PhD, Colorado State University.
References
  1. Liu Y, Zhao N, Kanemaki MT, Yamamoto Y, Sadamura Y, Ito Y, Tokunaga M, Stasevich TJ, Kimura H. Visualizing looping of two endogenous genomic loci using synthetic zinc-finger proteins with anti-FLAG and anti-HA frankenbodies in living cells. Genes Cells. 2021 Nov;26(11):905-926. doi: 10.1111/gtc.12893. Epub 2021 Sep 20. PMID: 34465007; PMCID: PMC8893316.
  2. Cialek CA, Galindo G, Morisaki T, Zhao N, Montgomery TA, Stasevich TJ. Imaging translational control by Argonaute with single-molecule resolution in live cells. Nat Commun. 2022 Jun 10;13(1):3345. doi: 10.1038/s41467-022-30976-3. PMID: 35688806; PMCID: PMC9187665.

If you publish research with this product, please let us know so we can cite your paper.

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