Anti-FPR1 [NFPRb] Antibody

This IgG mouse monoclonal was generated against human neutrophil N-formyl peptide receptor, NFPRb and is specific for FPR1.

Highlights:

  • Suitable for Western Blot, Immunoprecipitation, Immunofluorescence and Immunopurification applications

Formyl peptide receptors (FPR) are a family of G-protein-coupled receptors of mammalian phagocytic cells are involved in antibacterial host defense and inflammation. Activating these receptors can mediate neutrophil chemotaxis, production of reactive oxygen species and stimulate degranulation of neutrophils.

Also available: Anti-FPR1/2 [NFPRa] Antibody.

From the laboratory of Algirdas J. Jesaitis, PhD, Montana State University.

The Investigator's Annexe Part of The Investigator's Annexe program.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
EMT108
Anti-FPR1 [NFPRb] Antibody
100ug In stock
Regular Price:$338.00
On Sale:
Specifications

Product Type: Antibody
Antigen: NFPRb
Isotype: IgG1
Clonality: Monoclonal
Clone Name: NFPRb
Reactivity: Human
Specificity: Neutrophil and recombinant FPR1
Immunogen: Expressed and purified a hexa-histidine-tagged recombinant FPR1 (rHis-FPR1) in Spodoptera frugiperda(Sf9) cells
Species Immunized: Mouse
Myeloma Used: P3U1
Epitope: Cytoplasmic c-terminal tail residues 338-STLPSAEVELQAK-350
Buffer: 0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide
Tested Applications: WB, IF, IP (1:1000-4000), (2 -0.5?g/ml; depending on detection system)
Storage: -20C
Shipped: Cold packs

Data

Recognition of FPR1 and FPR2 in un-stimulated neutrophil homogenates and membranes

SDS-PAGE-separated and immunoblotted whole cell lysates (L) and salt-washed membranes (M) from N2 cavitates of unstimulated neutrophils, as described under “Experimental Procedures,” were developed with the NFPRb (left two lanes) and NFPRa (right two lanes) primary mAbs and visualized by infrared fluorescence scanning with a LI-COR Odyssey scanner. Each lane contains 2 × 105 cell equivalents. The broadly staining band between Mr ?50,000 and 65,000 (also known as 60K) is putatively FPR1 and is recognized by both mAbs. The band between Mr ?41,000 and 50,000 (also known as 45K) comigrates with FPR2 and is only recognized by NFPRa. The more intense bands at ?30,000 and ?36,000 are soluble cytosolic species and are lost in the particulate fraction.

Adapted from: Maaty WS, et al., J Biol Chem. 2013 Sep 20;288(38):27042-58.

Provider
From the laboratory of Algirdas J. Jesaitis, PhD, Montana State University.
References
  1. Maaty WS, Lord CI, Gripentrog JM, Riesselman M, Keren-Aviram G, Liu T, Dratz EA, Bothner B, Jesaitis AJ. Identification of C-terminal phosphorylation sites of N-formyl peptide receptor-1 (FPR1) in human blood neutrophils. J Biol Chem. 2013 Sep 20;288(38):27042-58.
  2. Schneider EH, Weaver JD, Gaur SS, Tripathi BK, Jesaitis AJ, Zelenka PS, Gao JL, Murphy PM. The leukocyte chemotactic receptor FPR1 is functionally expressed on human lens epithelial cells. J Biol Chem. 2012 Nov 23;287(48):40779-92.
  3. Riesselman M, Miettinen HM, Gripentrog JM, Lord CI, Mumey B, Dratz EA, Stie J, Taylor RM, Jesaitis AJ. C-terminal tail phosphorylation of N-formyl peptide receptor: differential recognition of two neutrophil chemoattractant receptors by monoclonal antibodies NFPR1 and NFPR2. J Immunol. 2007 Aug 15;179(4):2520-31.

If you publish research with this product, please let us know so we can cite your paper.

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