This IgG rabbit polyclonal antibody is reactive against human, mouse and rat IRX5.
Iroquouis homeobox (IRX) factors are a family of homeodomain transcription factors that contribute to many different cells in the body including brain, other organs and fat cells. Specifically, IRX5 (part of the Irxb subgroup) has be shown to be expressed in endocardium lining the ventricular and atrial myocardium.
From the laboratory of Chi-chung Hui, PhD, Hospital for Sick Children.
Part of The Investigator's Annexe program.
|Molecular Weight:||53 kDa|
|Reactivity:||Human, mouse and rat|
|Buffer:||Antiserum contains 0.02% Thimersol|
|Tested Applications:||WB (1:1000)|
Western Blot for Irx5 Expression
Western blot showing Irx5 expression in nuclear extract from epicardial myocardium (lane 1), endocardial myocardium (lane 2), isolated myocytes from epicardial myocardium (lane 3), isolated myocytes from endocardial myocardium (lane 4), isolated neonatal myocytes (lane 5), isolated myocytes from Irx5?/? epicardial myocardium (lane 6), and isolated myocytes from Irx5?/? endocardial myocardium (lane 7). GAPDH is shown as loading control.
Adapted from: Costantini DL, et al. Cell. 2005 Oct 21;123(2):347-58.
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