rMC-1 immortalized cells constitutively express GFAP (glial fibrillary acidic protein) and CRALBP (cellular retinaldehyde-binding protein) which are present in Müller cells.
Müller cells are the most abundant type of glial cells found in the vertebrate retina, and perform diverse functions that support the activity of retinal neurons. This immortalized cell line facilitates gene expression studies in Müller cells and improves the understanding of Müller cell-neuron interactions.
From the laboratory of Vijay Sarthy, PhD, Northwestern University.
|Product Type:||Cell Line|
|Cell Type:||Retinal Müller (glial) cell|
|Subculturing:||Harvest cells with 0.05% trypsin for 5 min and replate|
|Growth Conditions:||DMEM (high glucose, (+) L-Glutamine, (-) Sodium pyruvate) with 10% FBS and 1X pen/strep, with additional L-glutamine (2mM).|
|Cryopreservation:||DMEM with 10% DMSO and 10%FBS|
Morphology and immunocytochemical characterization of Müller cells.
(a) Primary culture of Müller cells. (b) Muller cell line, rMC-1. (c) Primary Müller cell culture stained with GFAP antibody. (d) rMC-1 reacted with GFAP antibody.
Adapted from: Sarthy, V. et al. Invest. Ophthalmol. Vis. Sci. 39: 212-216.
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