Vijay Sarthy, PhD, Northwestern University

Vijay Sarthy, PhD
Vijay Sarthy, PhD

The Sarthy lab focuses on gene regulation, development and functional organization of the vertebrate retina. Specifically, neuronal degeneration, injury, infection and trauma lead to cellular changes in neighboring glial cells. Some of the changes are related to wound healing and tissue repair, whereas others reflect glial cell involvement in protecting the neurons from further damage. In the vertebrate retina, rod and cone photoreceptor degeneration results in â??reactive gliosis' and up-regulation of certain genes in the neighboring Müller (glial) cells. A major goal of our current research work is to elucidate the molecular mechanisms responsible gene induction in â??reactive' Müller cells. Recent cell transfection studies and experiments with GFAP-lacZ transgenic mice have shown that the glial intermediate filament protein (GFAP) gene is strongly activated in â??reactive' Müller cells.

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References:

  1. Sarthy, V., S. Brodjian, K. Dutt, B. Kennedy, R. French and J. Crabb Establishment and characterization of a retinal Muller cell line. Invest. Ophthalmol. Vis. Sci. 1998 Jan;39: 212-216.
  2. Kannan R, Bao Y, Wang Y, Sarthy VP, Kaplowitz N.Protection from oxidant injury by sodium-dependent GSH uptake in retinal Müller cells. Exp Eye Res. 1999 May;68(5):609-16.
  3. Lu SC1, Bao Y, Huang ZZ, Sarthy VP, Kannan R. Regulation of gamma-glutamylcysteine synthetase subunit gene expression in retinal Müller cells by oxidative stress. Invest Ophthalmol Vis Sci. 1999 Jul;40(8):1776-82.
  4. Kusner LL, Sarthy VP, Mohr S. Nuclear translocation of glyceraldehyde-3-phosphate dehydrogenase: a role in high glucose-induced apoptosis in retinal Müller cells. Invest Ophthalmol Vis Sci. 2004 May;45(5):1553-61.
  5. Du Y, Sarthy VP, Kern TS. Interaction between NO and COX pathways in retinal cells exposed to elevated glucose and retina of diabetic rats. Am J Physiol Regul Integr Comp Physiol. 2004 Oct;287(4):R735-41.
  6. Loewen N, Chen J, Dudley VJ, Sarthy VP, Mathura JR Jr. Genomic response of hypoxic Müller cells involves the very low density lipoprotein receptor as part of an angiogenic network.Exp Eye Res. 2009 May;88(5):928-37.
  7. Xue W, Du P, Lin S, Dudley VJ, Hernandez MR, Sarthy VP. Gene expression changes in retinal Müller (glial) cells exposed to elevated pressure. Curr Eye Res. 2011 Aug;36(8):754-67.
  8. Muto T, Tien T, Kim D, Sarthy VP, Roy S. High glucose alters cx43 expression and gap junction intercellular communication in retinal müller cells: promotes müller cell and pericyte apoptosis. Invest Ophthalmol Vis Sci. 2014 Jun 17;55(7):4327-37.
  9. Tien T, Zhang J, Muto T, Kim D, Sarthy VP, Roy S. High Glucose Induces Mitochondrial Dysfunction in Retinal Müller Cells: Implications for Diabetic Retinopathy. Invest Ophthalmol Vis Sci. 2017 Jun 1;58(7):2915-2921. doi: 10.1167/iovs.16-21355. PubMed PMID: 28586916; PubMed Central PMCID: PMC5460955.