This IgG1 mouse mononoclonal antibody was generated against the partially purified human neutrophil membrane proteins and is reactive against human hCAP-18 amino acids 115-121 in the prodomain.
Cathelicidin-related antimicrobial peptides, are a family of polypeptides found in lysosomes of macrophages and polymorphoncuclear leukocytes (PMNs). Cathelicidins serve a critical role in mammalian innate immune defense against invasive bacterial infection. Specifically, human cathelicidin antimicrobial protein 18 (hCAP-18) is secreted from granules by activated neutrophils, to assist in wound healing, tissue repair and humoral immune responses. Cathelicidin antimicrobial peptides qualify as prototypes of innovative drugs that may be used to treat infection and/or regulate the immune response.
From the laboratory of Algirdas J. Jesaitis, PhD, Montana State University.
Part of The Investigator's Annexe program.
|Name:||Anti-Human Neutrophil CAP-18 [H7] Antibody|
|Molecular Weight:||16 kDa|
|Specificity:||Human hCAP-18 in pro domain|
|Immunogen:||Partially purified human neutrophil membrane proteins (heparin Ultrogel/lectin affinity purification fractions in octyl glucoside)|
|Purification Method:||Protein G|
|Buffer:||PBS, 0.05% (w/v) Sodium Azide|
|Tested Applications:||WB (1:1000), IF (10ug/mL), FACS (10ug/mL)|
Specific binding of mAb H7 to 16 and 14 kDa proteins present in the supernatant from fully degranulated neutrophils. Lane A, The 16-kDa hCAP-18 from intact, specific granules, purified by sucrose density gradient sedimentation of unstimulated neutrophils. Lane B,Supernatant from degranulated neutrophil supernatants. Lane C, Mixture (1:1) of samples shown in Lanes A and B.
Adapted from: Stie, J. et al. J.Leuk. Biol. 82:161-72. 2007.
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