Anti-Human Neutrophil CAP-18 [H7] Antibody

This IgG1 mouse mononoclonal antibody was generated against the partially purified human neutrophil membrane proteins and is reactive against human hCAP-18 amino acids 115-121 in the prodomain.

Cathelicidin-related antimicrobial peptides, are a family of polypeptides found in lysosomes of macrophages and polymorphoncuclear leukocytes (PMNs). Cathelicidins serve a critical role in mammalian innate immune defense against invasive bacterial infection. Specifically, human cathelicidin antimicrobial protein 18 (hCAP-18) is secreted from granules by activated neutrophils, to assist in wound healing, tissue repair and humoral immune responses. Cathelicidin antimicrobial peptides qualify as prototypes of innovative drugs that may be used to treat infection and/or regulate the immune response.

From the laboratory of Algirdas J. Jesaitis, PhD, Montana State University.

The Investigator's Annexe Part of The Investigator's Annexe program.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
EMT106
Anti-Human Neutrophil CAP-18 [H7] Antibody
100ug 4-6 weeks
Regular Price:$376.00
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Specifications

Product Type: Antibody
Name: Anti-Human Neutrophil CAP-18 [H7] Antibody
Molecular Weight: 16 kDa
Isotype: IgG1
Clonality: Monoclonal
Clone Name: H7
Specificity: Human hCAP-18 in pro domain
Reactivity: Human
Immunogen: Partially purified human neutrophil membrane proteins (heparin Ultrogel/lectin affinity purification fractions in octyl glucoside)
Species Immunized: Mouse
Myeloma Used: P3U1
Epitope: 115-NQARGSF-121
Purification Method: Protein G
Buffer: PBS, 0.05% (w/v) Sodium Azide
Tested Applications: WB (1:1000), IF (10ug/mL), FACS (10ug/mL)
Storage: -20C
Shipped: Cold packs

Data

Specific binding of mAb H7 to 16 and 14 kDa proteins present in the supernatant from fully degranulated neutrophils. Lane A, The 16-kDa hCAP-18 from intact, specific granules, purified by sucrose density gradient sedimentation of unstimulated neutrophils. Lane B,Supernatant from degranulated neutrophil supernatants. Lane C, Mixture (1:1) of samples shown in Lanes A and B.

Adapted from: Stie, J. et al. J.Leuk. Biol. 82:161-72. 2007.

Provider
From the laboratory of Algirdas J. Jesaitis, PhD, Montana State University.
References
  1. Stie, J. Jesaitis, A.V., Burritt, J.B., Taylor, R.M., Lord, C.I., Gripentrog, J.M. and Jesaitis, A.J.Localization of hCAP-18 on the Surface of Chemoattractant-stimulated Human Granulocytes: Analysis Using Two Novel hCAP-18-Specific Monoclonal Antibodies J.Leuk. Biol. 82:161-72. 2007.

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