Anti-p22phox [CS9] Antibody

This IgG1 mouse mononoclonal antibody was generated against the partially purified human neutrophil flavocytochrome b (cytochrome b559 or cytochrome b558) and is reactive against human p22phox amino acids 165-169 .

The integral membrane protein flavocytochrome b (Cyt b), a heterodimer formed by a 91-kD glycoprotein (heavy chain) and a 22-kD polypeptide (light chain), is the catalytic core of the phagocyte NADPH oxidase and generates superoxide which plays a critical role in host defense. The p22phox gene encodes the light alpha subunit which has been proposed as a primary component of the microbicidal oxidase system of phagocytes. Mutations in this gene are associated with autosomal recessive chronic granulomatous disease (CGD) that is characterized by the failure of activated phagocytes to generate superoxide, which is important for the microbicidal activity of these cells.

From the laboratory of Algirdas J. Jesaitis, PhD, Montana State University.

The Investigator's Annexe Part of The Investigator's Annexe program.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
EMT102
Anti-p22phox [CS9] Antibody
100ug 4-6 weeks
Regular Price:$435.00
On Sale:
Specifications

Product Type: Antibody
Name: Anti-p22phox [CS9] Antibody
Alternative Name(s): CYBA antibody, Cytochrome b 245 alpha polypeptide antibody, Cytochrome b 245 light chain antibody, Cytochrome b alpha polypeptide antibody, Cytochrome b(558) alpha chain antibody, Cytochrome b(558) alpha subunit antibody, Cytochrome b558 subunit alpha antibody, Flavocytochrome b 558 alpha polypeptide antibody, Neutrophil cytochrome b 22 kDa polypeptide antibody, p22 phagocyte B-cytochrome antibody, p22-phox antibody, p22phox antibody, Superoxide-generating NADPH oxidase light chain subunit antibody)
Accession ID: P13498
Molecular Weight: 21 kDa
Isotype: IgG1
Clonality: Monoclonal
Clone Name: b245
Specificity: Human p22phox
Reactivity: Human
Immunogen: Partially purified human neutrophil flavocytochrome b (heparin Ultrogel/lectin affinity purification - in Triton X-100)
Species Immunized: Mouse
Myeloma Used: P3U1
Epitope: 165-KKPSE-169
Purification Method: Protein G
Buffer: PBS, 0.05% (w/v) Sodium Azide
Tested Applications: WB (1:1000), IP (1:1000), FRET (1:1000), Immunopurification (1:1000)
Storage: -20C
Shipped: Cold packs

Provider
From the laboratory of Algirdas J. Jesaitis, PhD, Montana State University.
References
  1. Taylor, R.M, Burritt, J.B., Baniulis, D., Lord, C.I., Dinauer, M.C., Parkos, C.A. and Jesaitis, A.J. Characterization of Six Monoclonal Antibodies to the p22phox Subunit of Human Phagocyte Flavocytochrome b: Site Specific Inhibitors of NADPH Oxidase Activity and Probes for Analysis of Flavocytochrome b Surface Structure and Topology. J Immunol. 173:7349-57, 2004.
  2. Taylor, R.M. Jesaitis, A.J. Immunoaffinity purification of human phagocyte flavocytochrome b and analysis of conformational dynamics. Methods Mol Biol. 2007;412:429-37, 2007.

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