Immortalized rat skeletal muscle cells (L6) over-expresses GLUT4 with a myc-epitope, which is useful for measuring GLUT4 translocation.
Insulin stimulation of glucose transport in skeletal muscle results mainly due to translocation of the glucose transporter GLUT4 to the cell surface. L6 cells, originally derived from rat skeletal muscle, propagate as mononucleated myoblasts but can differentiate into multinucleated primary myotubes. The myotubes express several proteins typical of skeletal muscle including the GLUT4 glucose transporter. Insulin stimulates glucose uptake with high sensitivity and maximal responsiveness only in differentiated L6 myotubes and GLUT4 expression parallels the acquisition of these characteristics as the L6 cells differentiate. These features of L6 myotubes are important since GLUT4 is responsible for insulin-dependent glucose uptake in mature skeletal muscle.
Also available: L6 Rat Myoblast Cell Line
From the laboratory of Amira Klip, PhD, Hospital For Sick Children.
This product is for sale to Nonprofit customers only. For profit customers, please Contact Us for more information.
|Product Type:||Cell Line|
|Cell Type:||Skeletal muscle|
|Growth Conditions:||MEM-α (with Ribonucleosides and Deoxyribonucleosides) +10% FBS, 1% Antimycotic +5 ug/ml blasticidin|
|Cryopreservation:||MEM-α +10% FBS+ 10% DMSO|
|Comments:||The myc-epitope in the GLUT4myc has the amino acid sequence of AEEQKLISEEDLLK|
L6 myoblasts stably express rat GLUT4myc glucose transporter, a chimera of rat GLUT4 with human myc-epitope (AEEQKLISEEDLLK; recognized by monoclonal antibody clone 9E10) in the first exofacial loop of the transporter (between residue 66 and 67). Myoblasts should be passaged at approximately 70% of confluence. Myoblasts can be differentiated into myotubes by switching confluent myoblast monolayers to MEM-a +2% FBS for 4-5 days.*Antibiotic resistance for the GLUT4 stable transfection is blasticidin-HCl and passaging myoblasts with blasticidin in the growth media is recommended to maintain selection pressure.
Cell Line References
If you publish research with this product, please let us know so we can cite your paper.