Immortalized rat skeletal (L6) myoblast cell line that was selected for high fusion potential and endogenous expression of GLUT4 in the myotube stage.
Insulin stimulation of glucose transport in skeletal muscle results mainly due to translocation of the glucose transporter GLUT4 to the cell surface. L6 cells, originally derived from rat skeletal muscle, propagate as mononucleated myoblasts but can differentiate into multinucleated primary myotubes. The myotubes express several proteins typical of skeletal muscle including the GLUT4 glucose transporter. Insulin stimulates glucose uptake with high sensitivity and maximal responsiveness only in differentiated L6 myotubes and GLUT4 expression parallels the acquisition of these characteristics as the L6 cells differentiate. These features of L6 myotubes are important since GLUT4 is responsible for insulin-dependent glucose uptake in mature skeletal muscle.
Also available: L6-GLUT4myc Rat Myoblast Cell Line
From the laboratory of Amira Klip, PhD, Hospital For Sick Children.
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|Product Type:||Cell Line|
|Cell Type:||Skeletal muscle|
|Growth Conditions:||MEM-α (with Ribonucleosides and Deoxyribonucleosides) +10% FBS (See protocol below)|
|Cryopreservation:||MEM-α +10% FBS+ 10% DMSO|
Myoblasts should be passaged at approximately 70% of confluence. Myoblasts can be differentiated into myotubes by switching confluent myoblast monolayers to MEM-a +2% FBS for 4-5 days. Upon differentiation, the myotubes express more skeletal muscle proteins and based on our research, the GLUT4 glucose transporter, which is necessary for the development of insulin-stimulated glucose uptake. The insulin responsiveness of transporter-facilitated glucose uptake is ~2-fold in the myotube culture.
Cell Line References
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