Anti-ICOS (CD278) [C398.4A] Antibody

This hamster monoclonal antibody was generated against a mouse T helper cell clone, and recognizes human, mouse, and rat ICOS.

Inducible T-cell co-stimulator (ICOS) enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. The protein is essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. It does not up-regulate the production of interleukin-2, but superinduces the synthesis of interleukin-10. It prevents the apoptosis of pre-activated T-cells, and plays a critical role in CD40-mediated class switching of immunoglobin isotypes.

From the laboratory of Charles A. Janeway, Jr., MD, Yale University.

The Investigator's Annexe Part of The Investigator's Annexe program.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
Anti-ICOS (CD278) [C398.4A] Antibody
100ug 4-6 weeks
Regular Price:$335.00
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Product Type: Antibody
Antigen: Inducible T-cell co-stimulator (ICOS), CD278, AILIM, CVID1
Accession ID: Q9Y6W8
Molecular Weight: 34 kDa
Isotype: Hamster Ig (unknown isotype)
Clonality: Monoclonal
Clone Name: C398.4A
Reactivity: Mouse, rat, human
Immunogen: Mouse T helper cell clone
Species Immunized: Hamster
Purification Method: Protein G
Buffer: PBS, 0.05% (w/v) Sodium Azide
Tested Applications: IP, IHC, FC
Storage: -20C
Shipped: Cold packs



ICOS Glycosylation Patterns. Reduced immunoprecipitates of ICOS obtained from 125I-surface labeled HUT78 cell lysates were digested for the indicated times with Endo H. Lane C shows the undigested samples. Bold arrows mark the ICOS bands. Antibody C398.4A coupled to protein G-Sepharose was used. Samples were run on SDS-PAGE (14% polyacrylamide gels) under reducing conditionsand visualized with a Phospho imager.

Adapted from: Buonfiglio D, et al. Eur J Immunol. 2000 Dec;30(12):3463-7.


Immunohistochemical demonstration of hpH4 expression in angioimmunoblastic T cell lymphoma. Most neoplastic cells show cytoplasmic immunoreactivity for C398.4A mAb. Frozen sections; ABC method; hematoxylin counterstain. Original magnification 400 ×.

Adapted from: Buonfiglio D, et al. Eur J Immunol. 1999 Sep;29(9):2863-74.

Flow Cytometry

ICOS expression in L cells transfected with the human ICOS cDNA. ICOS-transfected (open curve) and wild-type cells (black curve) were stained with C398.4A followed by FITC-conjugated goat anti-mouse IgG or mouse anti-hamster IgG, respectively, and analyzed by flow cytometry.

Adapted from: Buonfiglio D, et al. Eur J Immunol. 2000 Dec;30(12):3463-7.

From the laboratory of Charles A. Janeway, Jr., MD, Yale University.
  • Redoglia V, Dianzani U, Rojo JM, Portolés P, Bragardo M, Wolff H, Buonfiglio D, Bonissoni S, Janeway CA Jr. Characterization of H4: a mouse T lymphocyte activation molecule functionally associated with the CD3/T cell receptor. Eur J Immunol. 1996 Nov;26(11):2781-9.
  • Buonfiglio D, Bragardo M, Bonissoni S, Redoglia V, Cauda R, Zupo S, Burgio VL, Wolff H, Franssila K, Gaidano G, Carbone A, Janeway CA Jr, Dianzani U. Characterization of a novel human surface molecule selectively expressed by mature thymocytes, activated T cells and subsets of T cell lymphomas. Eur J Immunol. 1999 Sep;29(9):2863-74.
  • Buonfiglio D, Bragardo M, Redoglia V, Vaschetto R, Bottarel F, Bonissoni S, Bensi T, Mezzatesta C, Janeway Jr CA, Dianzani U. The T cell activation molecule H4 and the CD28-like molecule ICOS are identical. Eur J Immunol. 2000 Dec;30(12):3463-7.

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