Anti-Endoglin (CD105) [44G4] Antibody

This mouse monoclonal antibody was generated against whole HOON Pre-B Leukemic Cells and recognizes human Endoglin (CD105).

Endoglin (CD105) is a homodimeric integral membrane glycoprotein comprised of a 95 kDa subunit. Endoglin is a component of the Transforming Growth Factor beta (TGF-ß) receptor system that functions as an ancillary receptor influencing binding of the TGF-ß family of ligands to signaling receptors. Endoglin binds to TGF-ß1 and TGF-ß3 with high affinity, but does not bind TGF-ß2. Endoglin is expressed by vascular endothelial cells and syncytiotrophoblasts of placenta and is weakly expressed by stromalfibroblasts. Endoglin is also expressed by U937 cells and by activated macrophages. Endoglin expression is up-regulated on activated endothelium in tissues undergoing angiogenesis, such as in tumors, or in cases of wound healing or dermal inflammation.

From the laboratory of Michelle Letarte, PhD, Hospital for Sick Children.

The Investigator's Annexe Part of The Investigator's Annexe program.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
Anti-Endoglin (CD105) [44G4] Antibody
100ug 4-6 weeks
Regular Price:$375.00
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Product Type: Antibody
Antigen: Endoglin (CD105)
Accession ID: P17813, NP_000109.1
Molecular Weight: 180 kDa
Isotype: IgG1k
Clonality: Monoclonal
Clone Name: 44G4
Reactivity: Human
Immunogen: ALL cell line (Whole HOON Pre-B Leukemic Cells)
Species Immunized: Mouse
Epitope: within mid portion of extracellular domain (Epitope: Tyr227-Gly331)
Purification Method: Protein G
Buffer: PBS, 0.05% (w/v) Sodium Azide
Tested Applications: Immunostaining on fresh sections, IP (1 ug/mL), WB (under non-reducing conditions only).
Storage: -20C
Shipped: Cold packs


Endoglin transiently expressed in COS-M6 cells binds TGF-beta 1.

COS-M6 cells were transfected with a cDNA encoding full-length endoglin (Endoglin) or control vector (C). Cells were affinity-labeled with 150 pM 125I-TGF-beta 1 and the detergent extracts incubated with mAb 44G4 followed by protein G-Sepharose. Immunoprecipitated proteins were analyzed by SDS-PAGE under reducing(R) and nonreducing (NR) conditions and visualized by autoradiography.

Adapted from: Cheifetz S, et al. J Biol Chem. 1992 Sep 25;267(27):19027-30.

From the laboratory of Michelle Letarte, PhD, Hospital for Sick Children.
  1. Quackenbush EJ, Letarte M. Identification of several cell surface proteins of non-T, non-B acute lymphoblastic leukemia by using monoclonal antibodies. J Immunol. 1985 Feb;134(2):1276-85.
  2. Quackenbush EJ, Gougos A, Baumal R, Letarte M. Differential localization within human kidney of five membrane proteins expressed on acute lymphoblastic leukemia cells. J Immunol. 1986 Jan;136(1):118-24.
  3. Cheifetz S, Bellón T, Calés C, Vera S, Bernabeu C, Massagué J, Letarte M. Endoglin is a component of the transforming growth factor-beta receptor system in human endothelial cells. J Biol Chem. 1992 Sep 25;267(27):19027-30.
  4. Gougos A, Letarte M. Primary structure of endoglin, an RGD-containing glycoprotein of human endothelial cells. J Biol Chem. 1990 May 25;265(15):8361-4.
  5. Gougos A, St Jacques S, Greaves A, O'Connell PJ, d'Apice AJ, Bühring HJ, Bernabeu C, van Mourik JA, Letarte M. Identification of distinct epitopes of endoglin, an RGD-containing glycoprotein of endothelial cells, leukemic cells, and syncytiotrophoblasts. Int Immunol. 1992 Jan;4(1):83-92.

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