This mouse monoclonal antibody was generated against whole HOON Pre-B Leukemic Cells and recognizes human Endoglin (CD105).
Endoglin (CD105) is a homodimeric integral membrane glycoprotein comprised of a 95 kDa subunit. Endoglin is a component of the Transforming Growth Factor beta (TGF-ß) receptor system that functions as an ancillary receptor influencing binding of the TGF-ß family of ligands to signaling receptors. Endoglin binds to TGF-ß1 and TGF-ß3 with high affinity, but does not bind TGF-ß2. Endoglin is expressed by vascular endothelial cells and syncytiotrophoblasts of placenta and is weakly expressed by stromalfibroblasts. Endoglin is also expressed by U937 cells and by activated macrophages. Endoglin expression is up-regulated on activated endothelium in tissues undergoing angiogenesis, such as in tumors, or in cases of wound healing or dermal inflammation.
From the laboratory of Michelle Letarte, PhD, Hospital for Sick Children.
Part of The Investigator's Annexe program.
|Accession ID:||P17813, NP_000109.1|
|Molecular Weight:||180 kDa|
|Immunogen:||ALL cell line (Whole HOON Pre-B Leukemic Cells)|
|Epitope:||within mid portion of extracellular domain (Epitope: Tyr227-Gly331)|
|Purification Method:||Protein G|
|Buffer:||PBS, 0.05% (w/v) Sodium Azide|
|Tested Applications:||Immunostaining on fresh sections, IP (1 ug/mL), WB (under non-reducing conditions only).|
Endoglin transiently expressed in COS-M6 cells binds TGF-beta 1.
COS-M6 cells were transfected with a cDNA encoding full-length endoglin (Endoglin) or control vector (C). Cells were affinity-labeled with 150 pM 125I-TGF-beta 1 and the detergent extracts incubated with mAb 44G4 followed by protein G-Sepharose. Immunoprecipitated proteins were analyzed by SDS-PAGE under reducing(R) and nonreducing (NR) conditions and visualized by autoradiography.
Adapted from: Cheifetz S, et al. J Biol Chem. 1992 Sep 25;267(27):19027-30.
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