This GFP reporter plasmid expresses CD44CR1, a noncoding sequence upstream of the CD44 locus.
CD44 is a glycoprotein that is ubiquitously expressed in most cells throughout the body and involved in cell adhesion, migration, differentiation, and survival. It has been found to be up regulated in various types of cancer stem cells, such as those of the prostate, pancreas, brain, and blood. CD44 overexpression in cancer stem cells leads to high resistance to chemotherapy and radiation treatment and an increase in metastasis.
From the laboratory of Li Cai, PhD, Rutgers University.
Part of The Investigator's Annexe program.
|Gene/insert name:||CD44CR1 ? a noncoding sequence upstream of CD44 locus|
|Format:||Liquid/spotted on filter paper|
|Grow in E. coli at 37 C:||Yes|
|Cloning Site 5':||SpeI|
|Cloning Site 3':||FseI|
|Insert Size:||715 bp|
|Vector Backbone and Size:||basal beta-globin promoter (bGP-GFP)|
|High or low copy:||High|
CR1 directs reporter GFP expression in breast cancer cell lines.
Conserved region was tested for the ability to direct reporter geneexpression by transfecting breast cancer cell lines with CD44CR1-bGP-GFP construct (CD44CR1-GFP). Nuclei were stained with Hoechst 33342. (AC) GFP expression in all three cell lines resulted from transfection of a positive control construct (CAG-GFP). (DF) No GFP expression was detected from transfection of a negative control construct with a conserved region from NeuroD1gene. GFP expression from CR1 can be seen in MDA-MB-231 and SUM159 cells (GH). However, no expression is seen in MCF7 cells (I).
Adapted from: Smith SM, Cai L. PLoS ONE 7(11): e50867.
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