This expression vector contains the core domain of mSA2 following an N-terminal 6xHis tag. The purification tag may be removed using TEV to yield mSA2 fused to a C-terminal FLAG tag.
Streptavidin is widely used in biotechnology and molecular research for detection, purification, crosslinking, and labeling of biotinylated targets. However, wild-type streptavidin is an obligate tetramer and can crosslink biotinylated targets. Target aggregation is a significant obstacle in some applications, including the labeling of biotinylated cell surface receptors, where crosslinking can perturb protein stability and function.
From the laboratory of Sheldon Park, PhD, University at Buffalo.
Part of The Investigator's Annexe program.
|Gene/insert name:||Monomeric Streptavidin (mSA) 2|
|Fusion Tag(s):||6xHis (N terminal on backbone), TEV (N terminal on insert), FLAG (C terminal on backbone)|
|Grow in E. coli at 37 C:||Yes|
|Cloning Site 5':||NheI|
|Cloning Site 3':||XhoI|
|Insert Size:||378 bp|
|Vector Backbone and Size:||pRSETA, 3566 bp|
|High or low copy:||High|
|Storage:||Store at -20C|
pRSET-mSA2 Vector Map
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