The HT103 monoclonal antibody was generated against an Influenza A whole virus and is specific for the nucleoprotein (NP) of all Influenza A viruses. The antibody has been tested via WB, ELISA, and IHC experiments.
The nucleoprotein (NP) of Influenza virus encapsulates the negative strand of the viral RNA and is essential for replicative transcription. It may also be involved in other essential functions throughout the virus life cycle. As well as binding ssRNA, NP is able to self associate to form large oligomeric complexes. NP is able to interact with a variety of other macromolecules of both viral and cellular origins. It binds the PB1 and PB2 subunits of the polymerase and the matrix protein 1 (M1).
From the laboratory of Thomas M. Moran, PhD, Icahn School of Medicine at Mount Sinai.
|Antigen:||Influenza A Nucleoprotein (NP)|
|Reactivity:||Binds NP of all viruses tested. Stains infected cells.|
|Purification Method:||Protein G|
|Buffer:||0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide|
|Tested Applications:||WB (1:200-1:1000), ELISA (1:200-1:1000), IHC (1:200-1:1000)|
Detection of NP in infected cells by Western blotting with the antibody HT103
Western blot analysis of MDCK cells infected with the recombinant cHA-expressing viruses. NOTE: anti-A/NP is HT103.
Adapted from: Hai, R., et al., J Virol 86:5774-5781.
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