Anti-Influenza A, Matrix Protein [E10] Antibody
![Anti-Influenza A, Matrix Protein [E10] Antibody](https://www.kerafast.com/MediaStorage/Product/Images/Large/756_200120200126207550.jpg "Anti-Influenza A, Matrix Protein [E10] Antibody")
The E10 monoclonal antibody was generated against a matrix protein 2 (M2) extracellular domain peptide-KLH conjugate and is specific for matrix proteins 1 and 2 (M1 and M2) of all Influenza A viruses. The antibody has been tested via WB, ELISA, IHC, and FCM experiments.
Highlights:
- Reacts with Influenza A Matrix Protein 1 (M1) and 2 (M2)
- Suitable for Western Blot, ELISA, Immunohistochemistry and Flow Cytometry applications
Recombinant versions available at our sister company, Absolute Antibody:
- Clone E10 available in various species, isotope and/or format
- Manufactured using Absolute Antibody’s Recombinant Platform with variable regions (i.e., specificity) from the hybridoma E10
Influenza A virus is an enveloped virus encoding 10 polypeptides. RNA segment 7 encodes for two proteins: matrix 1 (M1) and matrix 2 (M2). The M1 protein is critical for assembly and budding, interacting with the influenza hemagglutinin (HA) and neuraminidase (NA) proteins. M2 protein is a transmembrane protein composed of three domains: a 24 residue domain representing the N-terminal region, a 19 hydro-phobic residue domain that serve as a membrane anchor, and a 54 residue domain near the C-terminal in the cytoplasmic domain. The M2 protein has been found to play a role in Influenza replication and assembly of virion particles. It has been shown that this protein is an acid-activated ion channel for virus replication.
From the laboratory of Thomas M. Moran, PhD, Icahn School of Medicine at Mount Sinai.
The E10 monoclonal antibody was generated against a matrix protein 2 (M2) extracellular domain peptide-KLH conjugate and is specific for matrix proteins 1 and 2 (M1 and M2) of all Influenza A viruses. The antibody has been tested via WB, ELISA, IHC, and FCM experiments.
Highlights:
- Reacts with Influenza A Matrix Protein 1 (M1) and 2 (M2)
- Suitable for Western Blot, ELISA, Immunohistochemistry and Flow Cytometry applications
Recombinant versions available at our sister company, Absolute Antibody:
- Clone E10 available in various species, isotope and/or format
- Manufactured using Absolute Antibody’s Recombinant Platform with variable regions (i.e., specificity) from the hybridoma E10
Influenza A virus is an enveloped virus encoding 10 polypeptides. RNA segment 7 encodes for two proteins: matrix 1 (M1) and matrix 2 (M2). The M1 protein is critical for assembly and budding, interacting with the influenza hemagglutinin (HA) and neuraminidase (NA) proteins. M2 protein is a transmembrane protein composed of three domains: a 24 residue domain representing the N-terminal region, a 19 hydro-phobic residue domain that serve as a membrane anchor, and a 54 residue domain near the C-terminal in the cytoplasmic domain. The M2 protein has been found to play a role in Influenza replication and assembly of virion particles. It has been shown that this protein is an acid-activated ion channel for virus replication.
From the laboratory of Thomas M. Moran, PhD, Icahn School of Medicine at Mount Sinai.
| Product Type: | Antibody |
| Antigen: | Influenza A Matrix Protein 1 (M1) and 2 (M2) |
| Accession ID: | P05777 , P05780 |
| Molecular Weight: | 27.9 kDa (M1), 11.3 kDa (M2) |
| Isotype: | IgG2a |
| Clonality: | Monoclonal |
| Clone Name: | E10 |
| Reactivity: | Matrix protein 1 and 2; Stains cells infected with all Influenza viruses |
| Immunogen: | Matrix protein 2 (M2) extracellular domain peptide-KLH conjugate |
| Species Immunized: | Mouse |
| Purification Method: | Protein G |
| Buffer: | 0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide |
| Tested Applications: | WB (1:200-1:1000), ELISA (1:200-1:1000), IHC (1:200-1:1000), FCM (1:200-1:1000) |
| Storage: | -20C |
| Shipped: | Cold packs |
Detection of M1 and M2 proteins in infected cells by Western blotting with the antibody E10
Western blot of MDCK cells infected with either WSN or Mmut viruses (hpi: hours post-infection).
Adapted from: Bourmakina, S.V., et al., J Virol 79:7926-7932.
- Bourmakina, S.V., and A. Garcia-Sastre. 2005. The morphology and composition of influenza A virus particles are not affected by low levels of M1 and M2 proteins in infected cells. J Virol 79:7926-7932.
- Gannage, M., D. Dormann, R. Albrecht, J. Dengjel, T. Torossi, P.C. Ramer, M. Lee, T. Strowig, F. Arrey, G. Conenello, M. Pypaert, J. Andersen, A. Garcia-Sastre, and C. Munz. 2009. Matrix protein 2 of influenza A virus blocks autophagosome fusion with lysosomes. Cell Host Microbe 6:367-380.
- Yondola MA, Fernandes F, Belicha-Villanueva A, Uccelini M, Gao Q, Carter C, Palese P. Budding capability of the influenza virus neuraminidase can be modulated by tetherin. J Virol. 2011 Mar;85(6):2480-91.
- Tan GS, Krammer F, Eggink D, Kongchanagul A, Moran TM, Palese P. A pan-H1 anti-hemagglutinin monoclonal antibody with potent broad-spectrum efficacy in vivo. J Virol. 2012 Jun;86(11):6179-88.
- Ramos I, Carnero E, Bernal-Rubio D, Seibert CW, Westera L, García-Sastre A, Fernandez-Sesma A. Contribution of double-stranded RNA and CPSF30 binding domains of influenza virus NS1 to the inhibition of type I interferon production and activation of human dendritic cells. J Virol. 2013 Mar;87(5):2430-40.
- Henry Dunand CJ, Leon PE, Kaur K, Tan GS, Zheng NY, Andrews S, Huang M, Qu X, Huang Y, Salgado-Ferrer M, Ho IY, Taylor W, Hai R, Wrammert J, Ahmed R, García-Sastre A, Palese P, Krammer F, Wilson PC. Preexisting human antibodies neutralize recently emerged H7N9 influenza strains. J Clin Invest. 2015 Mar 2;125(3):1255-68.
- Voltersvik P, Aqrawi LA, Dudman S, Hungnes O; Norwegian Lung Pathology Group, Bostad L, Brokstad KA, Cox RJ. Pulmonary changes in Norwegian fatal cases of pandemic influenza H1N1 (2009) infection: a morphologic and molecular genetic study. Influenza Other Respir Viruses. 2016 Nov;10(6):525-531. View Article
- Calderon BM, Danzy S, Delima GK, et al. Dysregulation of M segment gene expression contributes to influenza A virus host restriction. PLoS Pathog. 2019;15(8):e1007892. Published 2019 Aug 15. View article
- Williams, G.D., Townsend, D., Wylie, K.M. et al. Nucleotide resolution mapping of influenza A virus nucleoprotein-RNA interactions reveals RNA features required for replication. Nat Commun 9, 465 (2018). View article
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