Anti-Influenza A, Matrix Protein [E10] Antibody

The E10 monoclonal antibody was generated against a matrix protein 2 (M2) extracellular domain peptide-KLH conjugate and is specific for matrix proteins 1 and 2 (M1 and M2) of all Influenza A viruses. The antibody has been tested via WB, ELISA, IHC, and FCM experiments.

Highlights:

  • Reacts with Influenza A Matrix Protein 1 (M1) and 2 (M2)
  • Suitable for Western Blot, ELISA, Immunohistochemistry and Flow Cytometry applications

Recombinant versions available at our sister company, Absolute Antibody:

Influenza A virus is an enveloped virus encoding 10 polypeptides. RNA segment 7 encodes for two proteins: matrix 1 (M1) and matrix 2 (M2). The M1 protein is critical for assembly and budding, interacting with the influenza hemagglutinin (HA) and neuraminidase (NA) proteins. M2 protein is a transmembrane protein composed of three domains: a 24 residue domain representing the N-terminal region, a 19 hydro-phobic residue domain that serve as a membrane anchor, and a 54 residue domain near the C-terminal in the cytoplasmic domain. The M2 protein has been found to play a role in Influenza replication and assembly of virion particles. It has been shown that this protein is an acid-activated ion channel for virus replication.

From the laboratory of Thomas M. Moran, PhD, Icahn School of Medicine at Mount Sinai.

Catalog Number Product Size AVAILABILITY Price Qty
EMS009
Anti-Influenza A, Matrix Protein [E10] Antibody
100ug In stock
Regular Price:$354.00
Specifications

Product Type: Antibody
Accession ID: P03485 (M1), P05780 (M2)
Antigen: Influenza A Matrix Protein 1 (M1) and 2 (M2)
Molecular Weight: 27.9 kDa (M1), 11.3 kDa (M2)
Isotype: IgG2a
Clonality: Monoclonal
Clone Name: E10
Reactivity: Matrix protein 1 and 2; Stains cells infected with all Influenza viruses
Immunogen: Matrix protein 2 (M2) extracellular domain peptide-KLH conjugate
Species Immunized: Mouse
Purification Method: Protein G
Buffer: 0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide
Tested Applications: WB (1:200-1:1000), ELISA (1:200-1:1000), IHC (1:200-1:1000), FCM (1:200-1:1000)
Storage: -20C
Shipped: Cold packs

Data

Detection of M1 and M2 proteins in infected cells by Western blotting with the antibody E10

E10 Figure

Western blot of MDCK cells infected with either WSN or Mmut viruses (hpi: hours post-infection).

Adapted from: Bourmakina, S.V., et al., J Virol 79:7926-7932.

Provider
From the laboratory of Thomas M. Moran, PhD, Icahn School of Medicine at Mount Sinai.
References
  1. Bourmakina, S.V., and A. Garcia-Sastre. 2005. The morphology and composition of influenza A virus particles are not affected by low levels of M1 and M2 proteins in infected cells. J Virol 79:7926-7932.
  2. Gannage, M., D. Dormann, R. Albrecht, J. Dengjel, T. Torossi, P.C. Ramer, M. Lee, T. Strowig, F. Arrey, G. Conenello, M. Pypaert, J. Andersen, A. Garcia-Sastre, and C. Munz. 2009. Matrix protein 2 of influenza A virus blocks autophagosome fusion with lysosomes. Cell Host Microbe 6:367-380.
  3. Yondola MA, Fernandes F, Belicha-Villanueva A, Uccelini M, Gao Q, Carter C, Palese P. Budding capability of the influenza virus neuraminidase can be modulated by tetherin. J Virol. 2011 Mar;85(6):2480-91.
  4. Tan GS, Krammer F, Eggink D, Kongchanagul A, Moran TM, Palese P. A pan-H1 anti-hemagglutinin monoclonal antibody with potent broad-spectrum efficacy in vivo. J Virol. 2012 Jun;86(11):6179-88.
  5. Ramos I, Carnero E, Bernal-Rubio D, Seibert CW, Westera L, García-Sastre A, Fernandez-Sesma A. Contribution of double-stranded RNA and CPSF30 binding domains of influenza virus NS1 to the inhibition of type I interferon production and activation of human dendritic cells. J Virol. 2013 Mar;87(5):2430-40.
  6. Henry Dunand CJ, Leon PE, Kaur K, Tan GS, Zheng NY, Andrews S, Huang M, Qu X, Huang Y, Salgado-Ferrer M, Ho IY, Taylor W, Hai R, Wrammert J, Ahmed R, García-Sastre A, Palese P, Krammer F, Wilson PC. Preexisting human antibodies neutralize recently emerged H7N9 influenza strains. J Clin Invest. 2015 Mar 2;125(3):1255-68.
  7. Voltersvik P, Aqrawi LA, Dudman S, Hungnes O; Norwegian Lung Pathology Group, Bostad L, Brokstad KA, Cox RJ. Pulmonary changes in Norwegian fatal cases of pandemic influenza H1N1 (2009) infection: a morphologic and molecular genetic study. Influenza Other Respir Viruses. 2016 Nov;10(6):525-531. View Article
  8. Calderon BM, Danzy S, Delima GK, et al. Dysregulation of M segment gene expression contributes to influenza A virus host restriction. PLoS Pathog. 2019;15(8):e1007892. Published 2019 Aug 15. View article
  9. Williams, G.D., Townsend, D., Wylie, K.M. et al. Nucleotide resolution mapping of influenza A virus nucleoprotein-RNA interactions reveals RNA features  required for replication. Nat Commun 9, 465 (2018). View article

If you publish research with this product, please let us know so we can cite your paper.

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