The E10 monoclonal antibody was generated against a matrix protein 2 (M2) extracellular domain peptide-KLH conjugate and is specific for matrix proteins 1 and 2 (M1 and M2) of all Influenza A viruses. The antibody has been tested via WB, ELISA, IHC, and FCM experiments.
Recombinant versions available at our sister company, Absolute Antibody:
Influenza A virus is an enveloped virus encoding 10 polypeptides. RNA segment 7 encodes for two proteins: matrix 1 (M1) and matrix 2 (M2). The M1 protein is critical for assembly and budding, interacting with the influenza hemagglutinin (HA) and neuraminidase (NA) proteins. M2 protein is a transmembrane protein composed of three domains: a 24 residue domain representing the N-terminal region, a 19 hydro-phobic residue domain that serve as a membrane anchor, and a 54 residue domain near the C-terminal in the cytoplasmic domain. The M2 protein has been found to play a role in Influenza replication and assembly of virion particles. It has been shown that this protein is an acid-activated ion channel for virus replication.
From the laboratory of Thomas M. Moran, PhD, Icahn School of Medicine at Mount Sinai.
|Accession ID:||P03485 (M1), P05780 (M2)|
|Antigen:||Influenza A Matrix Protein 1 (M1) and 2 (M2)|
|Molecular Weight:||27.9 kDa (M1), 11.3 kDa (M2)|
|Reactivity:||Matrix protein 1 and 2; Stains cells infected with all Influenza viruses|
|Immunogen:||Matrix protein 2 (M2) extracellular domain peptide-KLH conjugate|
|Purification Method:||Protein G|
|Buffer:||0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide|
|Tested Applications:||WB (1:200-1:1000), ELISA (1:200-1:1000), IHC (1:200-1:1000), FCM (1:200-1:1000)|
Detection of M1 and M2 proteins in infected cells by Western blotting with the antibody E10
Western blot of MDCK cells infected with either WSN or Mmut viruses (hpi: hours post-infection).
Adapted from: Bourmakina, S.V., et al., J Virol 79:7926-7932.
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