This mouse monoclonal antibody reacts with human, rabbit, and bovine Plectin (PLEC1); the antibody is specific for the C-terminal globular domain (aa 2,930-3,153). Immunoblotting experiments using cytoplasmic extracts from DJM-1 cells confirmed reactivity with the 500 kDa Plectin protein.
Plectin, present in nearly all mammalian cells, is a cytoskeletal liker protein whose size is approximetry 500kDa. Plectin is expressed in a wide variety of tissues, including skin, striated muscle, and gastrointestinal tract epithelia. Plectin acts as a link between the three main components of the cytoskeleton: actin microfilaments, microtubules and intermediate filaments. In addition, plectin links the cytoskeleton to junctions found in the plasma membrane that structurally connect different cells. By holding these different networks together plectin plays an important role in maintaining the mechanical integrity and viscoelastic properties of tissues. This protein is reported to relate to Epidermolysis Bullosa, Muscular Dystrophy and Pancreatic Ductal Adenocarcinoma.
From a laboratory at Nagoya University.
Part of The Investigator's Annexe program.
|Antigen:||Plectin (PLEC1), C-terminal Globular Domain|
|Molecular Weight:||Approx. 500 kDa|
|Reactivity:||Human, Rabbit, Bovine|
|Immunogen:||Recombinant His-tagged fusion protein of human Plectin, C-terminus (amino acids 2,930-3,153)|
|Buffer:||Cell culture supernatant; RPMI medium with 10% FCS and 0.02% sodium azide|
|Tested Applications:||Western blot (WB): starting dilution 1/10, dilution range 1/10-1/50Immunofluorescence (IF): starting dilution 1/10, dilution range 1/10-1/50|
|Storage:||-20C as an undilluted liquid for up to 12 months|
Immunofluorescence and Western Blot Analysis:
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