This affinity purified antibody was raised in rabbits against the C-terminal SRCAP peptide (SSAPPSLAGPAVSHRGRKAKT) and is recommended for detection of human SRCAP. It has been tested for use in Chromatin immunoprecipitation assays (ChIP), IP, and WB studies.
The SRCAP (SNF2-Related-CBP-Activator Protein) gene encodes the core catalytic component of the multiprotein chromatin-remodeling SRCAP complex. The encoded protein is an ATPase that is necessary for the incorporation of the histone variant H2A.Z into nucleosomes. It can function as a transcriptional activator in Notch-mediated, CREB-mediated and steroid receptor-mediated transcription. Mutations in this gene cause Floating-Harbor syndrome, a rare disorder characterized by short stature, language deficits and dysmorphic facial features.
From the laboratory of John Chrivia, PhD, St. Louis University.
Part of The Investigator's Annexe program.
|Accession ID:||Q6ZRS2, NP_006653|
|Molecular Weight:||400 kDa|
|Reactivity:||Human (use with other species has not been tested)|
|Epitope:||C-terminal SCRAP peptide (SSAPPSLAGPAVSHRGRKAKT)|
|Purification Method:||Affinity purified using anti-peptide column|
|Tested Applications:||IP (1:100), WB (1:1000), and ChIP assay (1:100)|
|Concentration:||1 mg/mL, (Bradford assay)|
Demonstration of WB and IP analyses.
SRCAP protein was immunoprecipitated from AA549 Nuclear extracts with (SLU1) or normal rabbit IgG and analyzed by Western blot using anti-SRCAP antibody (SLU2).
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