Anti-RIG-I [1C3] Antibody

This mouse IgG1 monoclonal antibody was generated against recombinant human RIG-I caspase recruitment domain (CARD), amino acids 1-211, and is specific for human RIG-I.

Retinoic acid-inducible gene 1 protein (RIG-I), also known as DEAD-box protein 58, is coded for by the DDX58 gene. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. RIG-I contains RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of innate immune responses. RIG-I is a cell receptor found in the cytoplasm of cells and functions as a pattern recognition receptor that is a sensor for viruses stimulating a type-I Interferon response through the mitochondrial protein IPS-1/MAVS.

From the laboratory of Adolfo Garcia-Sastre, PhD, Icahn School of Medicine at Mount Sinai.

 

Catalog Number Product Size AVAILABILITY Price Qty
EMS003
Anti-RIG-I [1C3] Antibody
100ug In stock
Regular Price:$317.00
Specifications

Product Type: Antibody
Name: Anti RIG-I monoclonal antibody (1C3)
Host: Mouse
Isotype: IgG1
Clonality: Monoclonal
Clone Name: 1C3
Specificity: Human RIG-I
Immunogen: Recombinant Human RIG-I caspase recruitment domain (CARD), amino acids 1-211
Format: Liquid
Purity: Protein G purified
Tested Applications: WB, IP (0.01 mg/mL cellular lysate)
Storage: -20C
Shipped: Cold packs

Provider
From the laboratory of Adolfo Garcia-Sastre, PhD, Icahn School of Medicine at Mount Sinai.
Comments

Western blot analysis from RIG-I pulldowns shows the efficiency of RIG-I immunoprecipitations.

Adapted from: Baum et al. Preference of RIG-I for short viral RNA molecules in infected cells revealed by next-generation sequencing. Proc Natl Acad Sci, 107, 16303, 2010.

References
  1. Baum et al. Preference of RIG-I for short viral RNA molecules ininfected cells revealed by next-generation sequencing. Proc Natl Acad Sci, 107, 16303, 2010.
  2. Nistal-Villan et al. Negative Role of RIG-I Serine 8 Phosphorylation in the Regulation of Interferon-beta Production. Journal of Biological Chemistry, 285, 20252, 2010.
  3. Durbin AF, Wang C, Marcotrigiano J, Gehrke L. RNAs Containing Modified Nucleotides Fail To Trigger RIG-I Conformational Changes for Innate Immune Signaling. MBio. 2016 Sep 20;7(5). pii: e00833-16. View Article
  4. Sato S, Li K, Kameyama T, Hayashi T, Ishida Y, Murakami S, Watanabe T, Iijima S, Sakurai Y, Watashi K, Tsutsumi S, Sato Y, Akita H, Wakita T, Rice CM, Harashima H, Kohara M, Tanaka Y, Takaoka A. The RNA sensor RIG-I dually functions as an innate sensor and direct antiviral factor for hepatitis B virus. Immunity. 2015 Jan 20;42(1):123-32. View Article
  5. Durbin AF, Wang C, Marcotrigiano J, Gehrke L. RNAs Containing Modified Nucleotides Fail To Trigger RIG-I Conformational Changes for Innate Immune Signaling. mBio. 2016;7(5):e00833-16. Published 2016 Sep 20. View Article

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