This mouse IgG1 monoclonal antibody was generated against recombinant human RIG-I caspase recruitment domain (CARD), amino acids 1-211, and is specific for human RIG-I.
Retinoic acid-inducible gene 1 protein (RIG-I), also known as DEAD-box protein 58, is coded for by the DDX58 gene. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. RIG-I contains RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of innate immune responses. RIG-I is a cell receptor found in the cytoplasm of cells and functions as a pattern recognition receptor that is a sensor for viruses stimulating a type-I Interferon response through the mitochondrial protein IPS-1/MAVS.
From the laboratory of Adolfo Garcia-Sastre, PhD, Icahn School of Medicine at Mount Sinai.
|Name:||Anti RIG-I monoclonal antibody (1C3)|
|Immunogen:||Recombinant Human RIG-I caspase recruitment domain (CARD), amino acids 1-211|
|Purity:||Protein G purified|
|Tested Applications:||WB, IP (0.01 mg/mL cellular lysate)|
Western blot analysis from RIG-I pulldowns shows the efficiency of RIG-I immunoprecipitations.
Adapted from: Baum et al. Preference of RIG-I for short viral RNA molecules in infected cells revealed by next-generation sequencing. Proc Natl Acad Sci, 107, 16303, 2010.
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