This recombinant FN fragment corresponds to amino acids S578-T778 of type-III 1-2 and contains the K638A and D736A mutations
Fibronectins (FN) are a family of high molecular weight, multidomain glycoproteins composed of two structurally similar subunits which are joined at the carboxyl terminus by disulfide bonds. The primary structure of FN is organized into three types of repeating homologous units, termed types I, II, and III. These modules in turn are organized into functional domains which have been shown to contain multiple binding sites, including those for sulfated glycosaminoglycans, gelatin, fibrin, and cell surface integrin receptors. Twelve type I modules are found grouped at the amino- and carboxyl-terminal regions, and two type II modules are located within the gelatin-binding region. Fifteen to seventeen type III modules are contained within the middle of the molecule and comprise 60% of fibronectin’s sequence.
From the laboratory of Denise C. Hocking, PhD, University of Rochester Medical Center.
Part of The Investigator's Annexe program.
|Name:||Recombinant Human Fibronectin III 1,2; amino acids S578-T778, with K638A and D736A mutations|
|Accession ID:||Q9H6D8, Q5VTL7, Q8TC99, Q8BJN4|
|Source:||Human protein expressed in E. coli DH5 alpha carrying the cloned gene in pGEX-2T|
|Molecular Weight:||48288.9 Da|
|Amino Acid Sequence:||SGPVEVFITETPSQPNSHPIQWNAPQPSHISKYILRWRPKNSVGRWKEATIPGHLNSYTIAGLKPGVVYEGQLISIQQYGHQEVTRFDFTTTSTSTPVTSNTVTGETTPFSPLVATSESVTEITASSFVVSWVSASDTVSGFRVEYELSEEGDEPQYLALPSTATSVNIPDLLPGRKYIVNVYQISEDGEQSLILSTSQTT|
|Fusion Tag(s):||GST, N-terminal|
|Purity:||> 90% by SDS-PAGE|
|Buffer:||Solution in PBS|
|Storage:||Store at -80C|
Human fibronectin subunits III 1-2 with mutations K638A and D736A
|FNIII 1-2 Solution Structure. Secondary structure elements are colored cyan and purple for FNIII 1 and FNIII 2, respectively. |
Adapted from: Vakonakis, I., Staunton, D., Rooney, L.M., and Campbell, I. D. (2007) Interdomain association in fibronectin: insight into cryptic sites and fibrillogenesis. EMBO J. 26: 2575-2583.
|Schematic representation of a fibronectin subunit and recombinant fibronectin fusion protein.|
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