pVSV-Δ-G-LASV-GP_nLucP Plasmid

The plasmid pVSVΔG-LASV-GP-NLucP encodes the antisense RNA of a replication competent chimeric vesicular stomatitis virus (rVSV) in which the VSV glycoprotein (G) has been replaced with the Lassa glycoprotein (GP). This encodes for a codon-optimized LASV glycoprotein Josiah strain. The genome also includes a nano-luciferasePEST reporter gene for easy monitoring of viral replication. This plasmid can be transfected into cells along with Helper Plasmids encoding the VSV nucleocapsid (N), phosphoprotein (P), glycoprotein (G), and large polymerase subunit (L) to recover rVSVΔG-LASV-GP-NLucP virus as described in [1-3].

Recombinant vesicular stomatitis virus (rVSVΔG) has been used to produce VSV chimeric viruses containing the envelope glycoproteins of heterologous viruses including viruses that require high-level containment. These particles are capable of multi-cycle replication.

It is the responsibility of the principal investigator to seek Institutional Biosafety Safety Committee approval for recombinant DNA, transgenic animal or infectious agent use within their laboratory spaces and maintain an Institutional Biosafety Safety Committee approval during the time period these materials are used.

From the laboratory of Melinda A. Brindley, PhD, University of Georgia.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
pVSV-Δ-G-LASV-GP_nLucP Plasmid
250ng In stock
Regular Price:$465.00
On Sale:

Product Type: Plasmid
Gene/insert name: VSVΔG-LASV-GP-NLucP
Fusion Tag(s): NLucP
Grow in E. coli at 37 C: Yes
Selectable markers: AmpR
Insert Size: 11883bp
Vector Backbone and Size: pBS-SK-ΦT, 3105bp
High or low copy: High
Storage: -20C
Shipped: Room temperature

From the laboratory of Melinda A. Brindley, PhD, University of Georgia.
  1. Whitt, M.A., Generation of VSV pseudotypes using recombinant DeltaG-VSV for studies on virus entry, identification of entry inhibitors, and immune responses to vaccines. J. Virol. Methods, 2010. 169(2): p. 365-74
  2. Lay Mendoza MF, Acciani MD, Levit CN, Santa Maria C, Brindley MA. Monitoring viral entry in real-time using a luciferase recombinant vesicular stomatitis virus producing SARS-CoV-2, EBOV, LASV, CHIKV, and VSV glycoproteins. Viruses. 2020 Dec 17; 12(12). PMID: 33348746
  3. Acciani MD, Lay-Mendoza MF, Havranek KE, Duncan AM, Iyer H, Linn OL, Brindley MA. Ebola virus requires phosphatidylserine scrambling activity for efficient budding and optimal infectivity. bioRxiv 2020.03.16.994012; doi:org/10.1101/2020.03.16.994012

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