DAP12 KO (C57) Macrophage Cell Line
A knockout DAP12 macrophage cell line isolated from bone marrow in C57BL/6 mice after immortalization with a retrovirus (J2) containing the myc and v-raf oncogenes.
DAP12 is transmembrane signaling polypeptide which contains an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. The encoded protein may associate with the killer-cell inhibitory receptor (KIR) family of membrane glycoproteins and may act as an activating signal transduction element. Mutations within this gene have been associated with polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL), also known as Nasu-Hakola disease. C57BL/6 also known as C57 black 6, C57 or black 6, is a common inbred strain of laboratory mouse. They are the most widely used and best-selling mouse strain, due to the availability of congenic strains, easy breeding, and robustness.
From the laboratory of Howard A. Young, PhD, National Cancer Institute/NIH.
A knockout DAP12 macrophage cell line isolated from bone marrow in C57BL/6 mice after immortalization with a retrovirus (J2) containing the myc and v-raf oncogenes.
DAP12 is transmembrane signaling polypeptide which contains an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. The encoded protein may associate with the killer-cell inhibitory receptor (KIR) family of membrane glycoproteins and may act as an activating signal transduction element. Mutations within this gene have been associated with polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL), also known as Nasu-Hakola disease. C57BL/6 also known as C57 black 6, C57 or black 6, is a common inbred strain of laboratory mouse. They are the most widely used and best-selling mouse strain, due to the availability of congenic strains, easy breeding, and robustness.
From the laboratory of Howard A. Young, PhD, National Cancer Institute/NIH.
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| Product Type: | Cell Line |
| Name: | DAP12 KO (C57) |
| Cell Type: | Macrophage |
| Accession ID: | O43914 |
| Morphology: | Adherent |
| Organism: | Mouse |
| Source: | Bone Marrow |
| Biosafety Level: | BSL2 |
| Growth Conditions: | DMEM + 10% FBS + Glutamine |
| Cryopreservation: | 90% FBS + 10% DMSO |
| Storage: | Liquid Nitrogen |
| Shipped: | Dry Ice |
- Blasi E, Radzioch D, Merletti L, Varesio L. Generation of macrophage cell line from fresh bone marrow cells with a myc/raf recombinant retrovirus. Cancer Biochem Biophys. 1989;10(4):303-317.
- Blasi E, Mathieson BJ, Varesio L, Cleveland JL, Borchert PA, Rapp UR. Selective immortalization of murine macrophages from fresh bone marrow by a raf/myc recombinant murine retrovirus. Nature. 1985;318(6047):667-670.
- Xiao C, Wang RH, Lahusen TJ, et al. Progression of chronic liver inflammation and fibrosis driven by activation of c-JUN signaling in Sirt6 mutant mice. J Biol Chem. 2012;287(50):41903-41913.
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