This fluorescent sulfonamide is used together with a fluorescent-labeled apocarbonic anhydrase sensor to measure and image free zinc ion in aqueous solution by wavelength ratio. These Fierke-Thompson sensors offer high sensitivity and selectivity, rapid response, ratiometric quantitation, cell penetration or expressibility in cells, targeting to desired organelles, and tunable zinc range and kinetics.
This cell-permeante fluorescent aryl sulfonamide is a component of excitation ratiometric fluorescence-based zinc biosensors employing human carbonic anhydrase II (CA II). It binds significantly more tightly to human CA II in the holo-form (Zn2+ in the active site) than in the apo-form; upon binding to holo-CA II it exhibits a 20-fold increase in fluorescence quantum yield and 60 nm emission blue shift (600 nm to 540 nm) compared to when free in aqueous solution. Extinction coefficient 21, 000 M-1 cm-1 at 370 nm. Its use with suitably fluorescent labeled hCA II for excitation ratiometric zinc determination is illustrated above. The sensor consists of suitably fluorescent-labeled apoCA II, (TAT-H36C-FLX-hCA wt-apoCA-tagRFP) together with this product; the protein may be expressed in a cell, or enter the cell membrane if it contains a cell-penetrant peptide such as TAT.
Read plog post from Dr. Richard Thompson at Pokegama Technologies, Improving Zinc Ion Quantitation with Unique Biosensor Proteins and Buffers.
|Product Type:||Small Molecule|
|Tested Applications:||Fluorescent zinc ion sensor|
|Storage:||Store frozen, dry, and tightly closed; millimolar solutions in DMF or DMSO generally are stable for months in the freezer|
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