pBG690 SARS-CoV-2 Nucleocapsid Protein Expression Plasmid

pBG690 SARS-CoV-2 Nucleocapsid Protein Expression Plasmid

Highlights:

  • Bacterial expression plasmid encodes a bacterial codon-optimized monomeric fragment of the SARS-CoV-2 nucleocapsid protein
  • Protein expressed and purified from this plasmid is antigenic
  • Protein can be used for ELISAs as well as other assays to indicate presence of SARS-CoV-2 nucleocapsid protein antibodies in patient samples

In response to the SARS-CoV-2/COVID-19 outbreak, the Geiss Lab produced a bacterial expression plasmid that encodes a bacterial codon-optimized fragment of the SARS-CoV-2 nucleocapsid protein. The SARS nucleocapsid protein was used as a diagnostic marker for SARS infection, and the recombinant SARS-CoV-2 nucleocapsid protein may be used as a similar diagnostic tool. To produce this plasmid, they synthesized a bacterial-codon optimized gene representing amino acids 133-416 of the SARS-CoV-2 nucleocapsid protein and cloned the sythentic gene into the pET28a T7 expression vector. The plasmid was sequence verified. They then developed a bacterial expression protocol that can produce monomeric nucleocapsid protein (as confirmed by mass spectroscopy) with greater than 95% purify. Purified nucleocapsid protein has been tested by collaborators for antigenicity in mice immunized against the recombinant protein as well as bats infected with SARS-CoV-2.

From the laboratory of Brian J. Geiss, PhD, Colorado State University.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
ECS010
pBG690 SARS-CoV-2 Nucleocapsid Protein Expression Plasmid
250ng (50ng/uL) In stock
Regular Price:$66.00
On Sale:
Specifications

Product Type: Plasmid
Name: SARS-CoV-2 Nucleocapsid Protein Expression Plasmid
Gene/insert name: SARS-CoV-2 Nucleocapsid Protein (aa 133-416)
Organism: E.coli
Antibiotic Resistance: Kanomycin
Fusion Tag(s): C-term His
Grow in E. coli at 37 C: Yes
Vector Backbone and Size: pET28a, 5369bp
High or low copy: High
Storage: -20C
Shipped: Room temperature

Provider
From the laboratory of Brian J. Geiss, PhD, Colorado State University.
References

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