pET 21d.1 VSV-M (M51R) plasmid

Vesicular stomatitis virus (VSV) is a well studied, enveloped, negative-strand RNA virus. The VSV genome encodes for 5 proteins: N, P, M, G, and L. The M protein (or matrix protein) is responsible for binding the nucleocapsid and condenses it into a tightly coiled helix and binds the nucleocapsid to the envelope. This activity of the M protein is what gives the virus its bullet like shape. In addition to M protein's role in virus assembly, it is also responsible for mediating molecular mechanisms of VSV pathogenesis. Wild-type M protein surpresses host gene expression in infected cells and inhibits antiviral responses. This activity is lost when the methionine at position 51 is substituted with an argenine (M51R). This M protein mutant maintains its ability to function in VSV virion assembly, but is unable to surpress host gene expression. This bacterial expression vector, encodes for M51R mutant M protein.

From the laboratory of Douglas S. Lyles, PhD, Wake Forest School of Medicine.

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Catalog Number Product DataSheet Size AVAILABILITY Price Qty
pET 21d.1 VSV-M (M51R) plasmid
Spotted on filter paper In stock
Regular Price:$115.00
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Product Type: Plasmid
Gene/insert name: VSV M-protein (M51R)
Alternative Name(s): Matrix protein
Accession ID: P03519
Antibiotic Resistance: Ampicilin
Fusion Tag(s): C-ter His-tag
Grow in E. coli at 37 C: yes
Insert Size: ~800bp
Vector Backbone and Size: pET21d, 5443bp
Shipped: Ambient temperature, spotted on filter paper

From the laboratory of Douglas S. Lyles, PhD, Wake Forest School of Medicine.
  1. Connor JH, McKenzie MO, Lyles DS. Role Residues 121 to 124 of Vesicular Stomatitis Virus Matrix Protein in Virus Assembly and Virus-Host Interactions. J. Virol. 2006; 80(8): p3701-3711.

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