Douglas S. Lyles, PhD, Wake Forest University

Douglas S. Lyles, Ph.D
Douglas S. Lyles, Ph.D.

Douglas S. Lyles laboratory studies the structure, assembly, and pathogenesis of enveloped RNA viruses. These projects are focused on vesicular stomatitis virus (VSV), a widely studied prototype enveloped virus that serves as a model for many other viruses that have envelopes as part of their structure. Research programs focus on molecular interactions involved in virus assembly and molecular mechanisms involved in viral pathogenesis. During studies of virus assembly, the laboratory discovered that the viral matrix protein suppresses host gene expression. This activity represents a second function for matrix protein in virus-infected cells in addition to its role in virus assembly. Research from his lab has led to new ideas for developing viral matrix protein mutants that selectively kill cancer cells (oncolytic viruses), and viral mutants that are more effective vaccine vectors.

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Recent Publications

  1. BraxtonCL, Puckett SH, Mizel SB, Lyles DS. Protection against lethal vacciniavirus challenge by using an attenuated matrix protein mutant vesicularstomatitis virus vaccine vector expressing poxvirus antigens. J Virol.2010; 84(7):3552-3561.
  2. KnellerELP, Connor JH, Lyles DS. hnRNPs relocalize to the cytoplasm followinginfection with vesicular stomatitis virus. J Virol. 2009; 83(2):770-780.
  3. AhmedM, Mitchell LM, Puckett S, Brzoza-Lewis KL, Lyles DS, Hiltbold EM.Vesicular stomatitis virus M protein mutant stimulates maturation ofToll-like receptor 7 (TLR7)-positive dendritic cells throughTLR-dependent and -independent mechanisms. J Virol. 2009;83(7):2962-2975.
  4. PearceAF, Lyles DS. Vesicular stomatitis virus induces apoptosis primarilythrough Bak rather than Bax by inactivating Mcl-1 and Bcl- XL. J Virol.2009; 83(18):9102-9112.
  5. DanchoB, McKenzie MO, Connor JH, Lyles DS. Vesicular stomatitis virus matrixprotein mutations that affect association with host membranes and viralnucleocapsids. J Biol Chem. 2009; 284(7):4500-4509.