Norbelladine 4'-O-Methyltransferase (NpN4OMT) from Narcissus sp. aff. pseudonarcissus is produced in E. coli BL21 (Rosetta) cells and purified by affinity chromatography using His-tag.
Norbelladine 4'-O-Methyltransferase (EC 18.104.22.1686) is an enzyme involved in Amaryllidaceae alkaloid biosynthesis that utilizes the co-substrate S-adenosyl methionine to methylate norbelladine to form 4'-O-methylnorbelladine.
From the laboratory of Toni M. Kutchan, PhD, and Megan Augustin, Donald Danforth Plant Science Center.
|EC Number:||EC 22.214.171.1246|
|Source:||E. coli BL21 (Rosetta)|
|Molecular Weight:||27,274 Da|
|Buffer:||50 mM potassium phosphate pH 8.0/ 10% glycerol|
|Comments:||Requires S-adenosyl methionine as co-substrate|
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