This murine fibroblast cell line is a derivative of the 4B6/3598/3442#2/4011C1 Cell Line (Cat. ESA104) and maintains mtDNA at a reduced copy number. To generate this cell line the parental cells, which maintain mtDNA copy number at normal levels were transiently transfected with a plasmid pMA4014, which encodes mCherry and FLPo and flow sorted for transfected cells. As a result of FLPo-mediated excision of the MTS in front of the bacterial LigA gene, mitochondrial import of LigA in 4B6/3598/3442#2/4011/4014C1-2 cells is inefficient, and mtDNA is maintained at a reduced copy number. This cell line in conjunction with its companion isogenic cell line 4B6/3598/3442#2/4011C1 (Cat. ESA104) is useful for studying the effects of mtDNA copy number on cellular physiology.
From the laboratory of Mikhail F Alexeyev, PhD, University of South Alabama.
|Product Type:||Cell Line|
|Cell Type:||Embryonic fibroblasts|
|Morphology:||Spindle-shaped at confluence|
|Source:||Mouse E13.5 Embryo|
|Biosafety Level:||BSL 1|
|Growth Conditions:||DMEM/10% FBS. Periodic selection with 10 µg/ml blasticidin, 2 µg/ml puromycin, and 1 mg/ml G418|
|Subculturing:||Split 1:4 to 1:8 every 3 days|
|Cryopreservation:||DMEM/10% FBS/10% DMSO|
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